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Gene Expression Data
Assay Details
Assay
Reference: J:130330 Ralston A, et al., Cdx2 acts downstream of cell polarization to cell-autonomously promote trophectoderm fate in the early mouse embryo. Dev Biol. 2008 Jan 15;313(2):614-29
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3777236
Gene symbol: Prkcz
Gene name: protein kinase C, zeta
Antibody: Anti-PKC zeta (sc-216; C-20)
Detection system: Secondary antibody
Results
Specimen 3A: embryonic day 2.0 (more )
Note: 4-cell embryo.
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Present 3A Expression is present but not polarized.
Specimen 3B: embryonic day 2.5 (more )
Note: 8-cell embryo.
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 3B Faint apical staining is detected most embryos at this stage.
Specimen 3C: embryonic day 3.0 (more )
Note: 16-cell embryo.
Structure Level Pattern Image Note
TS4: compacted morula Present 3C Expression is localized to the entire cell membrane of both inside and outside cells.
Specimen 3D: embryonic day 3.5 (more )
Note: 32-cell embryo.
Structure Level Pattern Image Note
TS4: blastocyst Present 3D Expression is localized to the entire cell membrane of both inside and outside cells.
Specimen 3F: embryonic day 3.5 (more )
Note: This is a blastocyst stage embryo.
Structure Level Pattern Image Note
TS4: trophectoderm Present Non-Uniform 3F There is expression at the apical surface.
Specimen 3G: embryonic day 3.5; Cdx2tm1Fbe/Cdx2tm1Fbe (more )
Note: This is a blastocyst stage embryo.
Structure Level Pattern Image Note
TS4: trophectoderm Present Non-Uniform 3G As in wild-type embryos, there is expression at the apical surface.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory