Probe:	clone 2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent

Structure	Level	Pattern	Image	Note
TS12: future midbrain neural fold	Present	Regionally restricted		Authors state that initial expression was detected in embryos with 6-7 somites, in scattered cells in the dorsal part of neural folds of the anterior midbrain.

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present	Regionally restricted	2A	At the 9-11 somite stage, midbrain expression is intensified and spread posteriorly.
TS13: future spinal cord	Present	Regionally restricted	2A	Expression also detected in the ventral spinal cord, between the junction with the hindbrain and the level of the forelimbs.

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Strong	Regionally restricted	2B	Expression also detected in the ventral spinal cord, between the junction with the hindbrain and the level of the forelimbs.
TS15: future forebrain	Present	Regionally restricted	2B	Other areas of expression included patches in the forebrain above the optic vesicle and in the nose area.
TS15: trunk mesenchyme derived from neural crest	Present		2B