Gene Expression Data
Assay Details
Assay
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Reference:
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J:130737
Tagariello A, et al., Ucma - A novel secreted factor represents a highly specific marker for distal chondrocytes. Matrix Biol. 2008 Jan;27(1):3-11
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3776651
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Gene symbol:
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Ucma
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Gene name:
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upper zone of growth plate and cartilage matrix associated
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Probe:
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Ucma probe1
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Probe preparation:
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Antisense labelled with P33 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
3B:
embryonic day 15.5
(more )
Specimen
3B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS23: radius |
Present |
Regionally restricted |
3B
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Expression is most prominent at the distal and proximal ends, in the distal chondrocytes.
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TS23: ulna |
Present |
Regionally restricted |
3B
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Expression is most prominent at the distal and proximal ends, in the distal chondrocytes.
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Specimen
3D:
embryonic day 16.5
(more )
Specimen
3D:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS24: radius |
Present |
Regionally restricted |
3D
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Expression is most prominent at the distal and proximal ends, in the distal chondrocytes.
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TS24: ulna |
Present |
Regionally restricted |
3D
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Expression is most prominent at the distal and proximal ends, in the distal chondrocytes.
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TS24: forelimb digit |
Weak |
Regionally restricted |
3D
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Expression is detectable in the distal chondrocytes.
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TS24: humerus |
Weak |
Regionally restricted |
3D
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Expression is detectable in the distal chondrocytes.
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Specimen
3E:
embryonic day 18.5
(more )
Specimen
3E:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS26: radius |
Present |
Regionally restricted |
3E
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Expression is most prominent at the distal and proximal ends, in the distal chondrocytes.
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TS26: ulna |
Present |
Regionally restricted |
3E
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Expression is most prominent at the distal and proximal ends, in the distal chondrocytes.
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TS26: forelimb digit |
Strong |
Regionally restricted |
3E
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Expression is detectable in the distal chondrocytes.
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TS26: humerus |
Strong |
Regionally restricted |
3E
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Expression is detectable in the distal chondrocytes.
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Specimen
5A:
embryonic day 16.5; Gli3Xt-J/Gli3 +
(more )
Specimen
5A:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Gli3Xt-J/Gli3+
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5B:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS24: forelimb skeleton |
Present |
Regionally restricted |
5B
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The expression domain in mutants is reduced compared to that seen in wild-type limbs.
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Specimen
5C:
embryonic day 16.5; Ihhtm1Amc/Ihh +
(more )
Specimen
5C:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Ihhtm1Amc/Ihh+
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5D:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Ihhtm1Amc/Ihhtm1Amc
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS24: forelimb skeleton |
Present |
Regionally restricted |
5D
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Expression is present in the outer cells surrounding the hypertrophic region.
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Specimen
5E:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J Ihhtm1Amc/Ihhtm1Amc
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS24: forelimb skeleton |
Present |
Regionally restricted |
5E
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Expression is restricted to the distal chondroyctes.
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Specimen
5G:
embryonic day 18.5; Ihhtm1Amc/Ihh +
(more )
Specimen
5G:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Ihhtm1Amc/Ihh+
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Age:
|
embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5H:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Ihhtm1Amc/Ihhtm1Amc
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Age:
|
embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: forelimb skeleton |
Present |
Regionally restricted |
5H
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Expression is present in the outer cells surrounding the hypertrophic region.
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