Probe:	2C-3
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS22: cardiac muscle tissue	Present	Regionally restricted	2AB	Variable levels of expression in the myocardium.
TS22: heart ventricle	Present	Regionally restricted	2AB	Highest levels of expression in basal half of ventricles.
TS22: right atrium	Present		2AB
TS22: heart	Strong	Regionally restricted	2AB	Strong expression in the atrioventricular ring, especially near the developing aortic, pulmonary and atrioventricular valve leaflets.
TS22: heart valve	Strong	Regionally restricted	2AB	Strong expression in the valve leaflets.
TS22: atrioventricular endocardial cushion	Strong		2AB
TS22: interatrial septum	Moderate		2AB
TS22: outflow tract aortic component	Present	Regionally restricted	2AB	Expression in mesenchyme surrounding the aorta.
TS22: vena cava	Present	Regionally restricted	2AB	Expression in the wall of the vena cava, in endothelial and/or smooth muscle cells, proximal to junction with atrium.
TS22: venous system	Present	Regionally restricted	2AB	Expression in endothelial and/or smooth muscle cells of pulmonary veins, proximal to junction with atrium.

Structure	Level	Pattern	Image	Note
TS22: ventricle cardiac muscle	Absent		2DE	No expression in the ventricular myocardium outside of the atrioventricular ring.
TS22: visceral pericardium	Weak	Regionally restricted	2DE	Weak expression in the epicardium, only in localized pockets.
TS22: outflow tract aortic component	Present	Regionally restricted	2DE	Expression in mesenchyme surrounding the aorta.
TS22: vena cava	Present	Regionally restricted	2DE	Expression in the wall of the vena cava, in endothelial and/or smooth muscle cells, proximal to junction with atrium.
TS22: venous system	Present	Regionally restricted	2DE	Expression in endothelial and/or smooth muscle cells of pulmonary veins, proximal to junction with atrium.

Structure	Level	Pattern	Image	Note
TS22: outflow tract pulmonary component	Present	Regionally restricted	2FG	Strong expression on pulmonary artery side of the valve leaflets. No expression in endothelial cells or smooth muscle cells of the pulmonary artery wall.

Structure	Level	Pattern	Image	Note
TS22: hepatic artery	Present	Regionally restricted		Expression in smooth muscle cells and, possibly, endothelial cells.

Structure	Level	Pattern	Image	Note
TS23: aortic valve leaflet	Present	Regionally restricted		Author report expression on the vascular side of leaflets in the aortic and pulmonary valves begins at E15.
TS23: pulmonary valve leaflet	Present	Regionally restricted		Author report expression on the vascular side of leaflets in the aortic and pulmonary valves begins at E15.

Structure	Level	Pattern	Image	Note
TS24: ventricle cardiac muscle	Weak	Regionally restricted	2HI	Diffuse weak expression in some of the ventricular myocardium.
TS24: heart	Strong	Regionally restricted	2HI	Strong expression in the atrioventricular ring.
TS24: aorta	Moderate	Regionally restricted	2HI	Moderate expression in mesenchyme surrounding the aorta.
TS24: heart atrium	Absent		2HI	No expression in the atrial wall.
TS24: visceral serosal pericardium	Absent		2HI	No expression in the epicardium.
TS24: endocardial tissue	Absent		2HI	No expression in the endocardium.

Structure	Level	Pattern	Image	Note
TS26: heart	Present	Regionally restricted		Authors report expression in the atrioventricular ring persists through E19.