Gene Expression Data
Assay Details
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Assay
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Reference:
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J:131019
Suzuki T, et al., Sequential expression of Efhc1/myoclonin1 in choroid plexus and ependymal cell cilia. Biochem Biophys Res Commun. 2008 Feb 29;367(1):226-33
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3775780
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Gene symbol:
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Efhc1
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Gene name:
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EF-hand domain (C-terminal) containing 1
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Antibody:
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myoclonin1 antibody
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Assay notes:
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The authors state that multiple different conditions for the fixation
of brain sections (pre-fixed frozen or fresh frozen) yielded no differences in the signals.
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Results
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Specimen
2A:
embryonic day 10.0
(more  )
Specimen
2A:
(close  )
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Genetic Background:
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either: C57BL/6J or ICR
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2B:
embryonic day 14.0
(more )
Specimen
2B:
(close )
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Genetic Background:
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either: C57BL/6J or ICR
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2C:
embryonic day 18.0
(more )
Specimen
2C:
(close )
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Genetic Background:
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either: C57BL/6J or ICR
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2D:
postnatal day 0
(more )
Specimen
2D:
(close )
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Genetic Background:
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either: C57BL/6J or ICR
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: brain ependyma |
Present |
Regionally restricted |
2D
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Expression is highly at cilia and moderately in somata of ependymal cells at the ventricles around hippocampus.
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Specimen
2E:
postnatal day 15
(more )
Specimen
2E:
(close )
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Genetic Background:
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either: C57BL/6J or ICR
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Age:
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postnatal day 15
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: brain ependyma |
Present |
Regionally restricted |
2E
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Expression is highly at cilia and moderately in somata of ependymal cells at the ventricles around hippocampus.
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Specimen
2F:
postnatal day 15
(more )
Note: This is a magnified view of 2E.
Specimen
2F:
(close )
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Genetic Background:
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either: C57BL/6J or ICR
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Age:
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postnatal day 15
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This is a magnified view of 2E.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: brain ependyma |
Present |
Regionally restricted |
2F
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Expression is highly at cilia and moderately in somata of ependymal cells at the ventricles around hippocampus.
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Specimen
2G:
postnatal day 15
(more )
Specimen
2G:
(close )
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Genetic Background:
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either: C57BL/6J or ICR
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Age:
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postnatal day 15
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: brain ependyma |
Present |
Regionally restricted |
2G
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Expression is in cells lining the wall of dorsal 3rd ventricle.
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Specimen
2H:
postnatal day 15
(more )
Note: This is a magnified view of 2G.
Specimen
2H:
(close )
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Genetic Background:
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either: C57BL/6J or ICR
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Age:
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postnatal day 15
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This is a magnified view of 2G.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: brain ependyma |
Present |
Regionally restricted |
2H
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Expression is in cells lining the wall of dorsal 3rd ventricle.
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Specimen
2I:
embryonic day 18.0
(more )
Specimen
2I:
(close )
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Genetic Background:
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either: C57BL/6J or ICR
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: lateral ventricle |
Present |
Regionally restricted |
2I
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Expression is in ependymal cells lining the lateral ventricle.
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Specimen
2J:
embryonic day 18.0
(more )
Specimen
2J:
(close )
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Genetic Background:
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either: C57BL/6J or ICR
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: 3rd ventricle |
Present |
Regionally restricted |
2J
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Expression is in cells lining the wall of dorsal 3rd ventricle.
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Specimen
2K:
embryonic day 18.0
(more )
Note: This is a magnified view of 2J.
Specimen
2K:
(close )
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Genetic Background:
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either: C57BL/6J or ICR
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This is a magnified view of 2J.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: 3rd ventricle |
Present |
Regionally restricted |
2K
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Expression is in cells lining the wall of dorsal 3rd ventricle.
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Specimen
2L:
embryonic day 18.0
(more )
Specimen
2L:
(close )
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Genetic Background:
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either: C57BL/6J or ICR
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: 4th ventricle |
Present |
Regionally restricted |
2L
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Expression is in cells lining the wall of dorsal 4th ventricle.
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Specimen
2M:
postnatal month 1
(more )
Specimen
2M:
(close )
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Genetic Background:
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either: C57BL/6J or ICR
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Age:
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postnatal month 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: brain ependyma |
Present |
Regionally restricted |
2M
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Expression is in somata and cilia of ependymal cells lining the wall of dorsal 3rd ventricle, ventral 3rd ventricle, and lateral ventricles.
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Specimen
2N:
postnatal month 1
(more )
Specimen
2N:
(close )
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Genetic Background:
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either: C57BL/6J or ICR
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Age:
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postnatal month 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: brain ependyma |
Present |
Regionally restricted |
2N
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Expression is in somata and cilia of ependymal cells lining the wall of dorsal 3rd ventricle, ventral 3rd ventricle, and lateral ventricles.
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Specimen
2O:
postnatal month 1
(more )
Specimen
2O:
(close )
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Genetic Background:
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either: C57BL/6J or ICR
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Age:
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postnatal month 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: brain ependyma |
Present |
Regionally restricted |
2O
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Expression is in somata and cilia of ependymal cells lining the wall of dorsal 3rd ventricle, ventral 3rd ventricle, and lateral ventricles.
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Specimen
2P:
postnatal month 1
(more )
Specimen
2P:
(close )
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Genetic Background:
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either: C57BL/6J or ICR
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Age:
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postnatal month 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: brain ependyma |
Present |
Regionally restricted |
2P
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Expression is in somata and cilia of ependymal cells lining the wall of dorsal 3rd ventricle, ventral 3rd ventricle, and lateral ventricles.
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Specimen
2Q:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Efhc1tm1Kzy/Efhc1tm1Kzy
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Age:
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postnatal month 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: brain ependyma |
Absent |
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2Q
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Expression is absent in somata and cilia of ependymal cells lining the wall of dorsal 3rd ventricle, ventral 3rd ventricle, and lateral ventricles.
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Specimen
2R:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Efhc1tm1Kzy/Efhc1tm1Kzy
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Age:
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postnatal month 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
|
Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: brain ependyma |
Absent |
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2R
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Expression is absent in somata and cilia of ependymal cells lining the wall of dorsal 3rd ventricle, ventral 3rd ventricle, and lateral ventricles.
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Specimen
2S:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Efhc1tm1Kzy/Efhc1tm1Kzy
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Age:
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postnatal month 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: brain ependyma |
Absent |
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2S
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Expression is absent in somata and cilia of ependymal cells lining the wall of dorsal 3rd ventricle, ventral 3rd ventricle, and lateral ventricles.
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Specimen
2T:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Efhc1tm1Kzy/Efhc1tm1Kzy
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Age:
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postnatal month 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: brain ependyma |
Absent |
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2T
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Expression is absent in somata and cilia of ependymal cells lining the wall of dorsal 3rd ventricle, ventral 3rd ventricle, and lateral ventricles.
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