Probe:	Sox9 cDNA3
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mesenchyme	Present		2a
TS15: head mesenchyme	Present		2a
TS15: otocyst	Present		2a
TS15: somite	Present	Regionally restricted	2a	Expression in rostral somites.
TS15: surface ectoderm	Present	Regionally restricted	2a	Expression in a scattered population of surface ectodermal cells overlying the spinal cord.

Structure	Level	Pattern	Image	Note
TS16: nose	Present	Regionally restricted	2b	Expression in nasal cartilage
TS16: somite	Present	Regionally restricted	2b	Expression in a discrete population of cells within the caudal somites
TS16: future forebrain	Present	Regionally restricted	2b	Expression in ventricular cells of the forebrain.

Structure	Level	Pattern	Image	Note
TS17: 2nd branchial arch	Present		2c
TS17: otocyst	Present		2c
TS17: trunk sclerotome	Present	Regionally restricted	2c	Expression in migrating sclerotomal cells.
TS17: limb mesenchyme	Present	Regionally restricted	2c	Expression in limb bud cartilage condensations
TS17: nose	Present	Regionally restricted	2c	Authors report expression in nasal cartilage.

Structure	Level	Pattern	Image	Note
TS19: future arm mesenchyme	Present	Regionally restricted	2e	Expression in the limb bud cartilage condensations, corresponding to radius, ulna and footplate.
TS19: handplate mesenchyme	Present	Regionally restricted	2e	Expression in the limb bud cartilage condensations, corresponding to radius, ulna and footplate.
TS19: skeleton	Present	Regionally restricted	2e	Expression in cartilage condensations corresponding to the scapula and pelvis.

Structure	Level	Pattern	Image	Note
TS20: cranium	Present	Regionally restricted	2f	Authors report expression in most skeletal structures, including the developing vertebrae, ribs, long bones, digits and cranial cartilage.
TS20: skeleton	Present	Regionally restricted	2f	Authors report expression in most skeletal structures, including the developing vertebrae, ribs, long bones, digits and cranial cartilage.
TS20: digit mesenchyme	Present		2f	Authors report expression in most skeletal structures, including the developing vertebrae, ribs, long bones, digits and cranial cartilage.
TS20: rib pre-cartilage condensation	Present		2f	Authors report expression in most skeletal structures, including the developing vertebrae, ribs, long bones, digits and cranial cartilage.
TS20: vertebral cartilage condensation	Present		2f	Authors report expression in most skeletal structures, including the developing vertebrae, ribs, long bones, digits and cranial cartilage.

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventricular layer	Present		2h	Expression in ventricular cells of the spinal cord.

Structure	Level	Pattern	Image	Note
TS21: axial skeleton tail region	Present	Regionally restricted	2i	Expression restricted to the tips of the digits, ribs, and to the tail tip vertebra;
TS21: limb digit	Present	Regionally restricted	2i	Expression restricted to the tips of the digits, ribs, and to the tail tip vertebra;
TS21: rib cartilage condensation	Present	Regionally restricted	2i	Expression restricted to the tips of the digits, ribs, and to the tail tip vertebra;
TS21: rib pre-cartilage condensation	Present	Regionally restricted	2i	Expression restricted to the tips of the digits, ribs, and to the tail tip vertebra;
TS21: digit mesenchyme	Present	Regionally restricted	2i	No expression in more mature cartilage., such as the long bones of the limbs and proximal parts of the digits.
TS21: limb cartilage condensation	Present	Regionally restricted	2i	No expression in more mature cartilage., such as the long bones of the limbs and proximal parts of the digits.
TS21: vibrissa	Present		2i	Expression in the vibrissae.