Probe:	Pax3 probe2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS14: prosencephalon lateral wall	Present		7F	In the head fold region, expression in the lateral neuroepithelium of the prosencephalon.
TS14: neural tube	Present		7F	In the trunk region, expression only just prior to closure of the neural tube; no expression in neural plate.
TS14: future spinal cord neural plate	Absent		7F	In the trunk region, expression only just prior to closure of the neural tube; no expression in neural plate.
TS14: trunk unsegmented mesenchyme	Absent		7F	No expression in presomitic mesoderm.
TS14: allantois	Absent		7F
TS14: primitive streak	Absent		7F

Structure	Level	Pattern	Image	Note
TS14: future spinal cord	Present	Regionally restricted	7G	Expression in a small population of cells in the dorsal region of the neural groove.
TS14: neural tube	Present		7G
TS14: somite	Present		7G	Expression in newly formed somites.

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Strong		7H	Strong expression in the developing dorsal root ganglia positioned between the neural tube and somitic mesoderm.
TS17: trunk dermomyotome	Present	Regionally restricted	7H	Segmental pattern of expression in the dermomytome, in both anterior and posterior halves of the somite, along the entire A-P axis of the embryo.

Structure	Level	Pattern	Image	Note
TS17: brain	Present	Regionally restricted	7I	Authors report expression as far anterior as the diencephalon.
TS17: neural tube	Present	Regionally restricted	7I	In the spinal cord, expression distributed throughout the dorsal half of the neural tube in both alar and root plates along the entire A-P axis of the embryo. Clear ventral border of expression coinciding with the sulcus limitans.
TS17: limb mesenchyme	Present	Regionally restricted	7I	Diffuse expression throughut distal limb bud mesenchyme.

Structure	Level	Pattern	Image	Note
TS19: metencephalon ventricular layer	Present		7J	Expression restricted to dorso-lateral walls of the mesencephalon, metencephalon and myencephalon, only in the ventricular layer.
TS19: midbrain ventricular layer	Present		7J	Expression restricted to dorso-lateral walls of the mesencephalon, metencephalon and myencephalon, only in the ventricular layer.
TS19: myelencephalon ventricular layer	Present		7J	Expression restricted to dorso-lateral walls of the mesencephalon, metencephalon and myencephalon, only in the ventricular layer.
TS19: future spinal cord	Present	Regionally restricted	7J	Sharp ventral border of expression in the spinal cord.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	7K	Sharp ventral border of expression in the spinal cord.

Structure	Level	Pattern	Image	Note
TS17: limb mesenchyme	Present	Regionally restricted	7L	Diffuse expression throughout distal limb bud mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: medulla oblongata ventricular layer	Present		7M	Expression in a thin layer of cells lining the ventricles of the metencephalon and myelencephalon
TS21: metencephalon ventricular layer	Present		7M	Expression in a thin layer of cells lining the ventricles of the metencephalon and myelencephalon
TS21: medulla oblongata part of 4th ventricle choroid plexus	Absent		7M	No expression in the developing choroid plexus derived from the roof plate of the fourth ventricle.
TS21: mandible	Present		7M
TS21: maxilla	Present		7M
TS21: nose	Present	Regionally restricted	7M	Expresssion in epithelial cells of the nasal pit (nasal process)

Structure	Level	Pattern	Image	Note
TS21: mandible	Present		7N
TS21: maxilla	Present		7N
TS21: nose	Present	Regionally restricted	7N	Expresssion in epithelial cells of the nasal pit (nasal process)

Structure	Level	Pattern	Image	Note
TS21: mandible	Present		7O
TS21: tongue	Present		7O
TS21: olfactory epithelium	Present		7O	Expresssion in epithelial cells of the nasal pit (olfactory/nasal epithelium)

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present	Regionally restricted	8A/B	High levels of expression throughout the dorsal part of the neural tube. Clear expression in regions from which neural crest cells will migrate.
TS14: trunk somite	Present		8A/B	Expression in dorso-laterally located mesoderm (somites) soon after closure of neural tube, prior to formation of the dermomyotome caps.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Strong	Regionally restricted	8C/D	High levels of expression throughout the dorsal part of the neural tube.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	8E/F	Sharp ventral border of expression maintained in the spinal cord, coinciding with the sulcus limitans.
TS20: spinal cord ventricular layer	Present		8E/F	Expression only in cells in the ventricular zone; no expression in intermediate zone cells.
TS20: dorsal root ganglion	Strong		8E/F

Structure	Level	Pattern	Image	Note
TS15: future spinal cord	Present	Regionally restricted	8G/H	In regions where the neural groove was just closing, expression in the dorsal-most cells of the neuroepithelium.
TS15: trunk mesenchyme	Absent		8G/H	No expression in mesoderm adjacent to the neuroepithelium.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	8I/J	Following closure of the neural tube, expression restricted initially to the dorsal neural tube.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	8K/L	More rostral sections showed expression in both dorsal neural tube and in the dorso-lateral region of adjacent somites.
TS15: trunk somite	Present	Regionally restricted	8K/L	More rostral sections showed expression in both dorsal neural tube and in the dorso-lateral region of adjacent somites.

Structure	Level	Pattern	Image	Note
TS20: medulla oblongata ventricular layer	Present	Regionally restricted	8A lower panel	In the myelencephalon, strong expression in cells throughout the ventricular zone of the dorso-lateral walls. Expression only in the ventricular zone; no expression in migrating neuroblasts.
TS20: medulla oblongata part of 4th ventricle choroid plexus	Absent		8A lower panel	No expression in the developing choroid plexus derived from the roof plate of the fourth ventricle.

Structure	Level	Pattern	Image	Note
TS20: medulla oblongata ventricular layer	Present	Regionally restricted	8B lower panel	In the myelencephalon, strong expression in cells throughout the ventricular zone of the dorso-lateral walls. Expression only in the ventricular zone; no expression in migrating neuroblasts.

Structure	Level	Pattern	Image	Note
TS20: head	Present	Regionally restricted	8C lower panel

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Present	Regionally restricted		Expression in the midbrain-hindbrain neuroepithelium.
TS14: future midbrain	Present	Regionally restricted		Expression in the midbrain-hindbrain neuroepithelium.
TS14: central nervous system	Present	Regionally restricted		Expression in the neuroepithelium extends almost continuously from the prosencephalon to the anterior margin of the posterior neuropore.
TS14: paraxial mesenchyme	Present	Regionally restricted		Expression in segmental mesoderm.

Structure	Level	Pattern	Image	Note
TS15: paraxial mesenchyme	Present	Regionally restricted		Expression in segmental mesoderm.

Structure	Level	Pattern	Image	Note
TS17: paraxial mesenchyme	Present	Regionally restricted		Expression in segmental mesoderm.
TS17: limb mesenchyme	Present			Expresssion in undifferentiated mesenchyme of forelimbs and hindlimbs.
TS17: spinal ganglion	Present
TS17: head mesenchyme derived from neural crest	Present			Expression in cranio-facial neural crest derivatives. Expression in cephalic neural crest cells in the nasal process.
TS17: 1st branchial arch mesenchyme derived from neural crest	Present			Expression in structures derived from the first and second branchial arches.
TS17: 2nd branchial arch mesenchyme derived from neural crest	Present			Expression in structures derived from the first and second branchial arches.

Structure	Level	Pattern	Image	Note
TS19: nasal pit epithelium	Present			Expression in epithelial cells of the nasal pit.
TS19: paraxial mesenchyme	Present	Regionally restricted		Expression in segmental mesoderm.
TS19: limb mesenchyme	Present			Expresssion in undifferentiated mesenchyme of forelimbs and hindlimbs.
TS19: spinal ganglion	Present
TS19: head mesenchyme derived from neural crest	Present			Expression in cranio-facial neural crest derivatives.
TS19: nasal process	Present	Regionally restricted		Expression in cephalic neural crest cells in the nasal process.
TS19: 1st branchial arch	Present	Regionally restricted		Expression in structures derived from the first and second branchial arches.
TS19: 2nd branchial arch	Present	Regionally restricted		Expression in structures derived from the first and second branchial arches.

Structure	Level	Pattern	Image	Note
TS20: medulla oblongata	Present	Regionally restricted		Sharp boundary of expression coinciding with the margin of the pyrimidal process that later forms the pyramid of the medulla oblongata.
TS20: midbrain lateral wall	Present	Regionally restricted		Expression in the dorso-lateral walls of the mesencephalon.
TS20: diencephalon	Present	Regionally restricted		Expression in a few cells at the dorsal midline of the posterior part of the diencephalon that will develop into the epithalamus.
TS20: epithalamus	Present			Expression in a few cells at the dorsal midline of the posterior part of the diencephalon that will develop into the epithalamus.
TS20: mandible primordium	Present
TS20: maxilla	Present
TS20: spinal ganglion	Present
TS20: tongue	Present
TS20: tail somite	Present			Expression in caudal-most somites.

Structure	Level	Pattern	Image	Note
TS21: mandible	Present
TS21: maxilla	Present
TS21: tongue	Present
TS21: tail somite	Present			Expression in caudal-most somites.

Structure	Level	Pattern	Image	Note
TS22: brain	Absent			No expression in anterior parts of the neural tube in either ventricular or intermediate zones.
TS22: tail spinal cord	Present	Regionally restricted		Expression persists in mitotically active cells in the caudal part of the neural tube.
TS22: tail somite	Present			Expression in caudal-most somites.