Gene Expression Data
Assay Details
Assay
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Reference:
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J:122362
Harwood BN, et al., Members of the WNT signaling pathways are widely expressed in mouse ovaries, oocytes, and cleavage stage embryos. Dev Dyn. 2008;237(4):1099-1111
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3775245
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Gene symbol:
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Dkk1
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Gene name:
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dickkopf WNT signaling pathway inhibitor 1
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Antibody:
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anti-Dkk-1
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Detection system:
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Secondary antibody coupled to Alexa Fluor 555
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Assay notes:
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Analysis of staining patterns was done on at least two biological replicates.
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Results
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Specimen
3:
postnatal day 21
(more )
Specimen
3:
(close )
Genetic Background:
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involves: C57BL/6J * DBA/2J
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Age:
|
postnatal day 21
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte |
Present |
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3_DKK1
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Expression is present in growing oocytes.
|
Specimen
Not shown:
postnatal
(more )
Specimen
Not shown:
(close )
Genetic Background:
|
involves: C57BL/6J * DBA/2J
|
Age:
|
postnatal
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte |
Present |
|
|
Expression is present in full-grown oocytes. Since transcripts were not detected in full-grown oocytes using RT-PCR, this suggests the protein is synthesized in the surrounding granulosa cells and is then transported to the oocytes.
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