Probe:	p53 cDNA8
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: embryo	Strong	Uniform	1A	Authors report strong expression in all tissues of the embryo.
TS17: extraembryonic component	Strong	Regionally restricted	1A	Strongest expression in the chorionic villi.
TS28: placenta	Present	Regionally restricted	1A	Within the maternal placenta, expression in epithelial cells lining the peripheral venous plexus.

Structure	Level	Pattern	Image	Note
TS20: liver	Strong	Regionally restricted	1B	Authors report expression in most liver cells, although intensity was heterogeneously distributed.
TS20: central nervous system	Strong		1B	Strong expression in all neuroepithelial cells.
TS20: nasal cavity	Present	Regionally restricted	1B	Clear expression in olfactory pits.

Structure	Level	Pattern	Image	Note
TS22: liver	Strong	Regionally restricted	1C	Authors report expression in most liver cells, although intensity was heterogeneously distributed.
TS22: bronchus epithelium	Strong		1C	Authors report high levels of expression in bronchial epithelia.
TS22: lung	Strong	Regionally restricted	1C	High levels of expression in lung submucosa

Structure	Level	Pattern	Image	Note
TS24: liver	Weak		1D	Rapid decline of expresssion in liver after day 14.5.
TS24: lung mesenchyme	Strong		1D	Strongest expression in mesodermal cells of lung
TS24: salivary gland	Present	Regionally restricted	1D	Higher levels of expression in salivary epithelium than in surrounding mesenchyme
TS24: hair follicle	Present	Regionally restricted	1D	Strong expression in ectodermal epithelia of external root sheath. Low levels of expression in primordia of dermal sheath.
TS24: hair root sheath	Present	Regionally restricted	1D	Strong expression in ectodermal epithelia of external root sheath. Low levels of expression in primordia of dermal sheath.
TS24: brown fat	Strong	Regionally restricted	1D	High levels of expression in pre-adipocytes.
TS24: brain ventricular layer	Strong		1D	Strongest expression in proliferating neuroepithelial cells surrounding ventricles of cerebellum, telencephalon and mesencephalon.
TS24: brain mantle layer	Present	Regionally restricted	1D	Moderate expression in outermost mantle zone.

Structure	Level	Pattern	Image	Note
TS17: yolk sac endoderm	Present	Regionally restricted	2	Moderate expression in visceral yolk sac endoderm.
TS17: extraembryonic component	Present	Regionally restricted	2	Prominent expression in blood-forming islands. Weak expression in Reichert's membrane. Authors state that the trophoblast secondary giant cells did not reveal strong hybridisation signals.

Structure	Level	Pattern	Image	Note
TS24: thymus	Strong		3A	High levels of expression in all cells of the thymus.

Structure	Level	Pattern	Image	Note
TS26: thymus	Present	Regionally restricted	3B	Expression is heterogeneously distributed.
TS26: thymus medullary core	Strong		3B	Strongest expression in large ovate reticulo-epithelial cells that form a compact stroma.

Structure	Level	Pattern	Image	Note
TS26: metanephros	Strong	Regionally restricted	4	Strong expression restricted to uninduced mesenchyme and newly formed epithelia in upper cortex.
TS26: glomerular tuft	Weak	Regionally restricted	4	Weak labelling in well-differentiated tubuli and glomeruli in central part of the kidney.

Structure	Level	Pattern	Image	Note
TS26: intestine epithelium	Strong	Regionally restricted	5	Stronger expression in epithelial cells within the intestinal crypts than in cells covering the villi.
TS26: intestine mesenchyme	Weak	Regionally restricted	5	Low levels of expression in mesodermal cells forming the submucosa.

Structure	Level	Pattern	Image	Note
TS26: enamel organ	Present	Regionally restricted	6	Strongest expression in outer layer of ameloblasts. Weak expression in cells of inner enamel epithelium.
TS26: dental papilla	Strong	Regionally restricted	6	Strong expression within the dental papillae.

Structure	Level	Pattern	Image	Note
TS26: hair follicle	Present	Regionally restricted	7
TS26: hair bulb	Strong		7	High levels of expression in epidermal hair bulb that partially surrounds the dermal papilla.

Structure	Level	Pattern	Image	Note
TS26: telencephalon	Present		8	Equal levels of expression in non-dividing outer neuroblast layer and ventricular layer.

Structure	Level	Pattern	Image	Note
TS24: vomeronasal organ	Strong		9	Strong expression in Jacobson's organ.
TS24: olfactory epithelium	Present		9	Moderate expression in olfactory epithelia.
TS24: vibrissa epidermal component	Strong	Regionally restricted	9	Strong expression in ectodermal epithelia of external root sheath.
TS24: vibrissa dermal component	Weak		9	Low levels of expression in primordia of dermal sheath.

Structure	Level	Pattern	Image	Note
TS12: embryo	Strong			Authors report strong expression in all tissues of the embryo.

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Strong			Strong expression in all neuroepithelial cells.`

Structure	Level	Pattern	Image	Note
TS20: trachea	Strong
TS20: lung mesenchyme	Present			Moderate expression in lung mesoderm

Structure	Level	Pattern	Image	Note
TS22: thymus primordium	Strong			High levels of expression in all cells of the thymus.
TS22: salivary gland epithelium	Strong			Higher levels of expression in salivary epithelium than in surrounding mesenchyme
TS22: salivary gland mesenchyme	Present			Higher levels of expression in salivary epithelium than in surrounding mesenchyme
TS22: hair follicle	Present	Regionally restricted		Low levels of expression in primordia of dermal sheath. Strong expression in ectodermal epithelia of external root sheath.

Structure	Level	Pattern	Image	Note
TS24: intestine epithelium	Strong	Regionally restricted		Stronger expression in epithelial cells within the intestinal crypts than in cells covering the villi.
TS24: intestine mesenchyme	Weak	Regionally restricted		Low levels of expression in mesodermal cells forming the submucosa.
TS24: enamel organ	Strong	Regionally restricted		Strongest expression in outer layer of ameloblasts. Weak expression in cells of inner enamel epithelium.
TS24: dental papilla	Strong			Strong expression within the dental papillae.
TS24: hair follicle	Present	Regionally restricted		High levels of expression in epidermal hair bulb that partially surrounds the dermal papilla.
TS24: hair bulb	Strong			High levels of expression in epidermal hair bulb that partially surrounds the dermal papilla.

Structure	Level	Pattern	Image	Note
TS26: lung	Weak			Expression in the lung was much weaker compared to earlier stages.
TS26: salivary gland epithelium	Weak			Greatly reduced expression in salivary gland epithelium.
TS26: brown fat	Weak			Reduced expression in brown fat compared with earlier stages.