Probe:	PRCi/l cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	The cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 20 min. before further processing.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present		1A PKC i/l	Expression is ubiquitous; there is no tissue-specific expression at this age.
TS28: placenta	Present		1A PKC i/l

Structure	Level	Pattern	Image	Note
TS13: embryo	Present			Expression is ubiquitous; there is no tissue-specific expression at this age.

Structure	Level	Pattern	Image	Note
TS15: embryo	Present			Expression is ubiquitous; there is no tissue-specific expression at this age.

Structure	Level	Pattern	Image	Note
TS17: embryo	Present		1B PKC i/l	Expression is ubiquitous; there is no tissue-specific expression at this age.

Structure	Level	Pattern	Image	Note
TS20: embryo	Present		2A	There is extensive expression throughout the embryo with regionalization in certain tissues.
TS20: corpus striatum	Strong		2A
TS20: midbrain	Strong		2A
TS20: olfactory epithelium	Strong		2C , 2A
TS20: neocortex	Strong		2A , 2E
TS20: choroid plexus	Strong		2A , 2G
TS20: medulla oblongata	Strong		2A , 2G
TS20: adenohypophysis	Strong		2A , 2I	There is strong expression in Rathke's pouch.
TS20: diencephalon	Strong		2I , 2A
TS20: liver	Strong		2A , 2K
TS20: lung	Present		2M , 2A	Expression is ubiquitous.
TS20: lobar bronchus	Present		2A , 2M	Expression levels are elevated in the segmental bronchi.
TS20: intestine	Strong		2O , 2A	There is strong expression in all layers.
TS20: metanephros	Present		2Q , 2A
TS20: stomach	Present		2Q , 2A
TS20: primitive bladder	Present		2S , 2A	Expression is present throughout the bladder.
TS20: urinary system	Present	Regionally restricted	2S , 2A	Expression is present in the urethra.

Structure	Level	Pattern	Image	Note
TS22: embryo	Present		3A	There is widespread expression throughout the embryo.
TS22: corpus striatum	Strong		3E , 3A
TS22: neocortex	Strong		3E , 3A
TS22: lateral ventricle choroid plexus	Strong		3A , 3E
TS22: trigeminal ganglion	Strong		3A
TS22: adenohypophysis	Strong		3I , 3A	Expression is present in Rathke's pouch.
TS22: diencephalon	Strong	Regionally restricted	3I , 3A	Expression is present in the diencephalon adjacent to Rathke's pouch.
TS22: medulla oblongata	Strong		3A
TS22: submandibular gland primordium	Present		3A
TS22: thymus primordium	Present		3M , 3A
TS22: dorsal root ganglion	Present		3A
TS22: spinal cord	Present		3A
TS22: cartilage	Present		3A	Expression is present in the cartilage primordium.
TS22: lung	Present		3Q , 3A	Expression is ubiquitous.
TS22: lobar bronchus	Present		3Q , 3A
TS22: mesenchyme of metanephros	Present		3U , 3A	Expression is present in the whole metanephron, including cortical, medullary, and mesenchymal regions.
TS22: metanephros	Present		3U , 3A	Expression is present in the whole metanephron, including cortical, medullary, and mesenchymal regions.
TS22: renal cortex	Present		3U , 3A	Expression is present in the whole metanephron, including cortical, medullary, and mesenchymal regions.
TS22: renal medulla	Present		3U , 3A	Expression is present in the whole metanephron, including cortical, medullary, and mesenchymal regions.
TS22: heart	Moderate		3A
TS22: liver	Moderate		3A , 3Q

Structure	Level	Pattern	Image	Note
TS22: embryo	Present		3C	There is widespread expression throughout the embryo.
TS22: olfactory epithelium	Strong		3K , 3C
TS22: cerebellum	Strong		3G , 3C
TS22: midbrain tegmentum	Strong		3G , 3C
TS22: superior colliculus	Strong		3G , 3C
TS22: medulla oblongata part of 4th ventricle choroid plexus	Strong		3G , 3C	High expression levels are detected in the choroid plexus within the fourth ventricle.
TS22: metencephalon part of 4th ventricle choroid plexus	Strong		3G , 3C	High expression levels are detected in the choroid plexus within the fourth ventricle.
TS22: adenohypophysis	Strong		3C	Expression is present in Rathke's pouch.
TS22: diencephalon	Strong	Regionally restricted	3C	Expression is present in the diencephalon adjacent to Rathke's pouch.
TS22: medulla oblongata	Strong		3C
TS22: thymus primordium	Present		3C
TS22: intestine	Present		3S , 3C , 3W	Expression is present throughout all layers.
TS22: urogenital sinus	Strong		3S , 3C
TS22: heart	Moderate		3C , 3O
TS22: liver	Moderate		3C

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present		4A , 4E
TS24: neocortex	Present	Regionally restricted	4E , 4A	Expression is present in the ventricular zone of the neocortex.
TS24: corpus striatum	Present		4A
TS24: olfactory epithelium	Present		4A , 4E
TS24: trigeminal ganglion	Present		4A
TS24: midbrain	Present	Regionally restricted	4A	Expression is present in the tectum and the segmentum of the midbrain.
TS24: medulla oblongata	Present		4A
TS24: choroid plexus	Weak		4A , 4E
TS24: adenohypophysis	Absent		4I , 4A	Expression is no longer present in Rathke's pouch.
TS24: submandibular gland	Strong		4A
TS24: thymus	Strong		4A
TS24: lung	Strong		4A , 4M , 4U	Expression is present in all tissues.
TS24: bronchiole epithelium	Trace		4A , 4U	Only marginal staining was observed in the epithelium of the main bronchus.
TS24: renal cortex	Present		4A , 4Q	Expression is present in both cortical and medullary regions.
TS24: renal medulla	Present		4A , 4Q	Expression is present in both cortical and medullary regions.
TS24: intestine epithelium	Present		4A	Expression is elevated in the epithelium at the base of the villi.
TS24: retina	Present		4Y1 , 4A
TS24: presumptive iris	Present	Regionally restricted	4Y1 , 4A	Expression is present in the cuboidal epithelium lining the iris.

Structure	Level	Pattern	Image	Note
TS24: olfactory epithelium	Present		4K , 4C
TS24: cerebellum	Present		4C
TS24: choroid plexus	Weak		4C
TS24: thymus	Strong		4C , 4O
TS24: spinal cord	Strong		4C , 4G
TS24: dorsal root ganglion	Strong		4C
TS24: brown fat	Strong		4C
TS24: intestine epithelium	Present		4C , 4S	Expression is elevated in the epithelium at the base of the villi.
TS24: bladder	Present		4C
TS24: testis	Present		4W , 4C	Expression is present throughout the testis.
TS24: epidermis	Present		4Z1 , 4C	Expression is present in all layers.
TS24: hair follicle	Present		4C , 4Z1

Structure	Level	Pattern	Image	Note
TS20: primitive bladder	Strong			Expression is ubiquitous.
TS20: cartilage	Strong			Expression is ubiquitous in the cartilage primordium.
TS20: choroid plexus	Strong			Expression is ubiquitous.
TS20: diencephalon	Strong			Expression is ubiquitous.
TS20: dorsal root ganglion	Strong			Expression is ubiquitous.
TS20: epidermis	Strong			Expression is ubiquitous.
TS20: heart	Strong			Expression is ubiquitous.
TS20: intestine	Strong			Expression is ubiquitous.
TS20: metanephros	Strong			Expression is ubiquitous.
TS20: liver	Strong			Expression is ubiquitous.
TS20: lung	Strong			Expression is ubiquitous.
TS20: medulla oblongata	Strong			Expression is ubiquitous.
TS20: midbrain	Strong			Expression is ubiquitous.
TS20: neocortex	Moderate			Expression is ubiquitous.
TS20: olfactory epithelium	Strong			Expression is ubiquitous.
TS20: adenohypophysis	Strong			Expression is ubiquitous in Rathke's pouch.
TS20: spinal cord	Strong			Expression is ubiquitous.
TS20: urinary system	Present	Regionally restricted		Expression is present in the urethra.

Structure	Level	Pattern	Image	Note
TS22: bladder	Present	Regionally restricted		Expression levels vary from strong to low.
TS22: cartilage	Strong			Expression is ubiquitous in the cartilage primordium.
TS22: cerebellum	Strong			Expression is ubiquitous.
TS22: choroid plexus	Strong			Expression is ubiquitous.
TS22: diencephalon	Strong			Expression is ubiquitous.
TS22: dorsal root ganglion	Strong			Expression is ubiquitous.
TS22: epidermis	Strong			Expression is ubiquitous.
TS22: heart	Moderate			Expression is ubiquitous.
TS22: intestine	Strong			Expression is ubiquitous.
TS22: metanephros	Strong			Expression is ubiquitous.
TS22: liver	Moderate			Expression is ubiquitous.
TS22: lung	Strong			Expression is ubiquitous.
TS22: medulla oblongata	Strong			Expression is ubiquitous.
TS22: midbrain	Strong			Expression is ubiquitous.
TS22: neocortex	Strong			Expression is ubiquitous.
TS22: olfactory epithelium	Strong			Expression is ubiquitous.
TS22: pancreas	Absent
TS22: adenohypophysis	Strong			Expression is ubiquitous in Rathke's pouch.
TS22: spinal cord	Strong			Expression is ubiquitous.
TS22: submandibular gland primordium	Strong			Expression is ubiquitous.
TS22: thymus primordium	Strong			Expression is ubiquitous.

Structure	Level	Pattern	Image	Note
TS24: bladder	Present	Regionally restricted		Expression levels vary from strong to low.
TS24: cartilage	Moderate			Expression is ubiquitous in the cartilage primordium.
TS24: cerebellum	Moderate			Expression is ubiquitous.
TS24: choroid plexus	Weak			Expression is ubiquitous.
TS24: diencephalon	Moderate			Expression is ubiquitous.
TS24: dorsal root ganglion	Strong			Expression is ubiquitous.
TS24: epidermis	Strong			Expression is ubiquitous.
TS24: eye	Present	Regionally restricted		Expression levels vary from medium to strong.
TS24: heart	Moderate			Expression is ubiquitous.
TS24: intestine	Moderate	Regionally restricted
TS24: metanephros	Strong			Expression is ubiquitous.
TS24: liver	Weak			Expression is ubiquitous.
TS24: lung	Strong			Expression is ubiquitous.
TS24: medulla oblongata	Moderate			Expression is ubiquitous.
TS24: midbrain	Moderate			Expression is ubiquitous.
TS24: neocortex	Strong	Regionally restricted
TS24: olfactory epithelium	Strong			Expression is ubiquitous.
TS24: pancreas	Absent
TS24: adenohypophysis	Trace			Expression is present in Rathke's pouch.
TS24: spinal cord	Moderate			Expression is ubiquitous.
TS24: submandibular gland	Strong			Expression is ubiquitous.
TS24: testis	Strong			Expression is ubiquitous.
TS24: thymus	Strong			Expression is ubiquitous.