Probe:	MH1116 probe 941
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Strong		3B Sdhb
TS22: midbrain	Weak		3B Sdhb
TS22: cerebellum	Weak		3B Sdhb
TS22: pons	Moderate		3B Sdhb
TS22: medulla oblongata	Moderate		3B Sdhb
TS22: spinal cord	Moderate		3B Sdhb
TS22: cranial ganglion	Moderate		3B Sdhb
TS22: dorsal root ganglion	Moderate		3B Sdhb
TS22: olfactory epithelium	Weak		3B Sdhb
TS22: intestine	Weak		3B Sdhb
TS22: salivary gland	Moderate		3B Sdhb
TS22: tongue	Weak		3B Sdhb
TS22: stomach	Weak		3B Sdhb
TS22: liver	Moderate		3B Sdhb
TS22: lung	Moderate		3B Sdhb
TS22: metanephros	Moderate		3B Sdhb
TS22: thyroid gland	Moderate		3B Sdhb
TS22: pituitary gland	Weak		3B Sdhb
TS22: pancreas	Weak		3B Sdhb
TS22: adrenal gland	Weak		3B Sdhb
TS22: heart	Moderate		3B Sdhb
TS22: skeletal muscle	Moderate		3B Sdhb
TS22: cartilage	Absent		3B Sdhb	There is no expression in the axial cartilage.
TS22: skeleton	Absent		3B Sdhb	There is no expression in the long bones.

Structure	Level	Pattern	Image	Note
TS22: embryo	Present		MH1116_3B , MH1116_4B	There is a stereotypical energy metabolic enzyme pattern of expression. Expression levels are fairly similar everywhere.