Probe:	MH1299 probe 1068
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Moderate		3B Idh2
TS22: midbrain	Moderate		3B Idh2
TS22: cerebellum	Weak		3B Idh2
TS22: pons	Moderate		3B Idh2
TS22: medulla oblongata	Moderate		3B Idh2
TS22: spinal cord	Moderate		3B Idh2
TS22: cranial ganglion	Weak		3B Idh2
TS22: dorsal root ganglion	Moderate		3B Idh2
TS22: olfactory epithelium	Moderate		3B Idh2
TS22: intestine	Strong		3B Idh2
TS22: salivary gland	Moderate		3B Idh2
TS22: tongue	Strong		3B Idh2
TS22: stomach	Moderate		3B Idh2
TS22: liver	Moderate		3B Idh2
TS22: lung	Strong		3B Idh2
TS22: metanephros	Moderate		3B Idh2
TS22: thyroid gland	Moderate		3B Idh2
TS22: pituitary gland	Moderate		3B Idh2
TS22: pancreas	Strong		3B Idh2
TS22: adrenal gland	Moderate		3B Idh2
TS22: heart	Strong		3B Idh2
TS22: skeletal muscle	Moderate		3B Idh2
TS22: cartilage	Absent		3B Idh2	There is no expression in the axial cartilage.
TS22: skeleton	Absent		3B Idh2	There is no expression in the long bones.

Structure	Level	Pattern	Image	Note
TS22: embryo	Present		MH1299_3D , MH1299_1B	There is nearly ubiquitous expression in the embryo.
TS22: choroid plexus	Present		MH1299_1B , MH1299_3D	This is one of the tissues with the highest expression level.
TS22: heart	Present		MH1299_1B , MH1299_3D	This is one of the tissues with the highest expression level.
TS22: lens	Present		MH1299_1B , MH1299_3D	This is one of the tissues with the highest expression level.
TS22: skeletal muscle	Present		MH1299_1B , MH1299_3D	This is one of the tissues with the highest expression level.
TS22: skeleton	Absent		MH1299_3D , MH1299_1B	There is no expression in the developing axial and appendicular cartilage.