Probe:	MH1408 probe 1130
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Moderate		3B Cs
TS22: midbrain	Weak		3B Cs
TS22: cerebellum	Weak		3B Cs
TS22: pons	Moderate		3B Cs
TS22: medulla oblongata	Weak		3B Cs
TS22: spinal cord	Weak		3B Cs
TS22: cranial ganglion	Strong		3B Cs
TS22: dorsal root ganglion	Strong		3B Cs
TS22: olfactory epithelium	Moderate		3B Cs
TS22: intestine	Weak		3B Cs
TS22: salivary gland	Strong		3B Cs
TS22: tongue	Weak		3B Cs
TS22: stomach	Weak		3B Cs
TS22: liver	Moderate		3B Cs
TS22: lung	Moderate		3B Cs
TS22: metanephros	Moderate		3B Cs
TS22: thyroid gland	Weak		3B Cs
TS22: pituitary gland	Weak		3B Cs
TS22: pancreas	Weak		3B Cs
TS22: adrenal gland	Weak		3B Cs
TS22: heart	Moderate		3B Cs
TS22: skeletal muscle	Weak		3B Cs
TS22: cartilage	Absent		3B Cs	There is no expression in the axial cartilage.
TS22: skeleton	Absent		3B Cs	There is no expression in the long bones.

Structure	Level	Pattern	Image	Note
TS22: cranial ganglion	Present		MH1408_5A , MH1408_3A	This is one of the tissues with the highest level of expression.
TS22: dorsal root ganglion	Present		MH1408_5A , MH1408_3A	This is one of the tissues with the highest level of expression.
TS22: salivary gland	Present		MH1408_5A , MH1408_3A	This is one of the tissues with the highest level of expression.
TS22: vibrissa follicle	Present		MH1408_5A , MH1408_3A	This is one of the tissues with the highest level of expression.
TS22: main olfactory bulb	Present	Regionally restricted	MH1408_3A , MH1408_5A	Expression is present in differentiating neurons.
TS22: cranial nerve	Present	Regionally restricted	MH1408_5A , MH1408_3A	Expression is present in a subset of cranial nerve nuclei.
TS22: heart	Present		MH1408_5A , MH1408_3A
TS22: liver	Present		MH1408_5A , MH1408_3A
TS22: lung	Present		MH1408_5A , MH1408_3A
TS22: metanephros	Present		MH1408_5A , MH1408_3A
TS22: thymus primordium	Present		MH1408_5A , MH1408_3A