Probe:	MH2056 probe 1820
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Moderate		3A Eno1
TS22: midbrain	Moderate		3A Eno1
TS22: cerebellum	Moderate		3A Eno1
TS22: pons	Moderate		3A Eno1
TS22: medulla oblongata	Moderate		3A Eno1
TS22: spinal cord	Moderate		3A Eno1
TS22: cranial ganglion	Moderate		3A Eno1
TS22: dorsal root ganglion	Moderate		3A Eno1
TS22: olfactory epithelium	Moderate		3A Eno1
TS22: intestine	Moderate		3A Eno1
TS22: salivary gland	Moderate		3A Eno1
TS22: tongue	Moderate		3A Eno1
TS22: stomach	Moderate		3A Eno1
TS22: liver	Moderate		3A Eno1
TS22: lung	Moderate		3A Eno1
TS22: metanephros	Moderate		3A Eno1
TS22: thyroid gland	Moderate		3A Eno1
TS22: pituitary gland	Moderate		3A Eno1
TS22: pancreas	Moderate		3A Eno1
TS22: adrenal gland	Moderate		3A Eno1
TS22: heart	Moderate		3A Eno1
TS22: skeletal muscle	Weak		3A Eno1
TS22: cartilage	Absent		3A Eno1	There is no expression in the axial cartilage.
TS22: skeleton	Absent		3A Eno1	There is no expression in the long bones.

Structure	Level	Pattern	Image	Note
TS22: integumental system	Strong		MH2056_4B , MH2056_3B
TS22: embryo	Present	Regionally restricted	MH2056_4B , MH2056_3B	Except for the strong expression in the integuments and the lack of expression in the prevertebrae, all tissues express weakly.
TS22: vertebral pre-cartilage condensation	Absent		MH2056_3B , MH2056_4B