Gene Expression Data
Assay Details
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Assay
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Reference:
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J:123677
Li X, et al., Mouse Pachytene Checkpoint 2 (Trip13) Is Required for Completing Meiotic Recombination but Not Synapsis. PLoS Genet. 2007 Aug 10;3(8):e130
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3769037
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Gene symbol:
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Trip13
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Gene name:
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thyroid hormone receptor interactor 13
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Antibody:
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anti-TRIP13
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
1F WT:
postnatal adult
(more  )
Specimen
1F WT:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: testis |
Present |
Regionally restricted |
1F WT
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| TS28: spermatocyte |
Strong |
Single cells |
1F WT
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Expression most prominent in the nuclei of Type B spermatogonia and leptotene spermatocytes; less strong expression in zygotene/early pachytene spermatocytes. No expression in late pachytene spermatocytes.
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| TS28: spermatogonium |
Strong |
Single cells |
1F WT
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Expression most prominent in the nuclei of Type B spermatogonia and leptotene spermatocytes; less strong expression in zygotene/early pachytene spermatocytes. No expression in late pachytene spermatocytes.
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Specimen
1F MUT:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Trip13Gt(RRB047)Byg/Trip13Gt(RRB047)Byg
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: testis |
Absent |
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1F MUT
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No nuclear staining was seen in mutant testis sections.
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Analysis Tools