Gene Expression Data
Assay Details
Assay
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Reference:
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J:1337
Blum M, et al., Gastrulation in the mouse: the role of the homeobox gene goosecoid. Cell. 1992 Jun 26;69(7):1097-106
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3768133
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Gene symbol:
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Gsc
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Gene name:
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goosecoid homeobox
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Probe:
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Probe 2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
4A:
embryonic day 6.4
(more )
Note: Egg cylinder stage.
Specimen
4A:
(close )
Genetic Background:
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(C57BL/6J x SJL/J)F1
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Age:
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embryonic day 6.4
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Egg cylinder stage.
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Structure |
Level |
Pattern |
Image |
Note |
TS9: embryo |
Present |
Regionally restricted |
4A
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Expression in a circular patch in the embryonic epiblast.
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Specimen
4B:
embryonic day 6.6
(more )
Note: Early primitive streak stage.
Specimen
4B:
(close )
Genetic Background:
|
(C57BL/6J x SJL/J)F1
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Age:
|
embryonic day 6.6
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
Note:
|
Early primitive streak stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS9: primitive streak |
Present |
Regionally restricted |
4B
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Expression at the anterior end of the primitive streak.
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Specimen
4C:
embryonic day 6.7
(more )
Specimen
4C:
(close )
Genetic Background:
|
(C57BL/6J x SJL/J)F1
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Age:
|
embryonic day 6.7
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Age Note:
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Age of embryo at noon of plug day not specified in reference. Embryos were also staged by morphological criteria.
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS9: embryo |
Present |
Regionally restricted |
4C
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Patch of expression localized to tip of embryo.
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Specimen
5A:
embryonic day 6.8
(more )
Specimen
5A:
(close )
Genetic Background:
|
(C57BL/6J x SJL/J)F1
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Age:
|
embryonic day 6.8
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Age Note:
|
Age of embryo at noon of plug day not specified in reference. Embryos were also staged by morphological criteria.
|
Sex:
|
Not Specified
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Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS9: embryo |
Present |
Regionally restricted |
5A
|
Expression at tip of egg cylinder, restricted to the mesodermal layer at the anterior end of the primitive streak.
|
TS9: primitive streak |
Present |
Regionally restricted |
5A
|
Expression at tip of egg cylinder, restricted to the mesodermal layer at the anterior end of the primitive streak.
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Specimen
4D:
embryonic day 6.9
(more )
Specimen
4D:
(close )
Genetic Background:
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(C57BL/6J x SJL/J)F1
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Age:
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embryonic day 6.9
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Age Note:
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Age of embryo at noon of plug day not specified in reference. Embryos were also staged by morphological criteria.
|
Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
|