Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:1337
Blum M, et al., Gastrulation in the mouse: the role of the homeobox gene goosecoid. Cell. 1992 Jun 26;69(7):1097-106
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:3768132
|
Gene symbol:
|
Gsc
|
Gene name:
|
goosecoid homeobox
|
Probe:
|
Probe 2
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
Assay notes:
|
Authors report that probe 2 and probe 1 (MGI:14167) gave qualitatively the same results but background was considerably higher with probe 1 (not shown).
|
|
Results
|
Specimen
3A:
embryonic day 6.5
(more )
Specimen
3A:
(close )
Genetic Background:
|
(C57BL/6J x SJL/J)F1
|
Age:
|
embryonic day 6.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Structure |
Level |
Pattern |
Image |
Note |
TS9: embryo |
Present |
Regionally restricted |
3A
|
Expression in a patch of cells of the epiblast on one side of the egg cylinder, corresponding to the site where the primitive streak was befinning to form.
|
Specimen
3B:
embryonic day 6.5
(more )
Specimen
3B:
(close )
Genetic Background:
|
(C57BL/6J x SJL/J)F1
|
Age:
|
embryonic day 6.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Structure |
Level |
Pattern |
Image |
Note |
TS9: embryo |
Present |
Regionally restricted |
3B
|
Expression in a patch of cells of the epiblast on one side of the egg cylinder, corresponding to the site where the primitive streak was befinning to form.
|
Specimen
3C:
embryonic day 6.5
(more )
Specimen
3C:
(close )
Genetic Background:
|
(C57BL/6J x SJL/J)F1
|
Age:
|
embryonic day 6.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Structure |
Level |
Pattern |
Image |
Note |
TS9: embryo |
Present |
Regionally restricted |
3C
|
Expression within the posterior half of the egg cylinder.
|
TS9: primitive streak |
Present |
|
3C
|
|
Specimen
3D:
embryonic day 7.0
(more )
Note: Mid-streak stage.
Specimen
3D:
(close )
Genetic Background:
|
(C57BL/6J x SJL/J)F1
|
Age:
|
embryonic day 7.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Bouin's Fixative
|
Note:
|
Mid-streak stage.
|
|