Tg(Il10-Thy1a)1Weav
Transgene Detail
|
|
| Symbol: |
Tg(Il10-Thy1a)1Weav |
| Name: |
transgene insertion 1, Casey T Weaver |
| MGI ID: |
MGI:3767675 |
| Synonyms: |
10BiT, Il10.Thy1.1 BAC-In Transgenic |
| Transgene: |
Tg(Il10-Thy1a)1Weav Location: unknown
|
| Alliance: |
Tg(Il10-Thy1a)1Weav page
|
|
|
|
| Transgene Type: |
|
Transgenic (Reporter) |
| Mutation: |
|
Insertion
|
| |
|
Mutation details: A transgene consisting of the coding sequence of Thy1a (Thy1.1, CD90.1) followed by SV40 poly A sequence was inserted into a mouse Il10 gene contained in a BAC. The reporter cassette was inserted one codon after the ATG start site and a premature stop codon was included after the poly A signal to prevent downstream expression of the Il10gene. The modified BAC, including 113 kb of genomic DNA upstream and 83kb genomic DNA downstream of the reporter cassette, was injected into single-cell embryos. One founder line containing 12 copies of the reporter transgene was selected for further study. Transgene expression was confirmed in T cells cultured under Th2 polarizing conditions.
(J:125255)
|
|
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
|
|
On a C57BL/6 background, expression of the Thy1a transgene can be differentiated from endogenous Thy1 expression by an allele specific antibody.
|
| Original: |
J:124255 Maynard CL, et al., Regulatory T cells expressing interleukin 10 develop from Foxp3(+) and Foxp3(-) precursor cells in the absence of interleukin 10. Nat Immunol. 2007 Sep;8(9):931-41 |
| All: |
44 reference(s) |
|
Analysis Tools
