Probe:	FG25 probe 7
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Weak	Regionally restricted	FG25	There is weak expression in the intermediate zone throughout the telencephalon.
TS22: inferior colliculus	Weak	Single cells	FG25
TS22: spinal cord	Weak	Regionally restricted	FG25	Expression is present in the ventral aspect of the spinal cord.
TS22: lung	Present		FG25
TS22: salivary gland	Present		FG25
TS22: pancreas	Absent		FG25	Although others have reported expression in the pancreas using RT-PCR, these authors were unable to detect it using in situ hybridization.