Probe:	FG37 probe 24
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Absent		3D

Structure	Level	Pattern	Image	Note
TS22: thymus primordium	Absent		3H

Structure	Level	Pattern	Image	Note
TS22: rib cartilage condensation	Absent		3L	There is no expression in the proliferating chondrocytes in the core region of the ribs.

Structure	Level	Pattern	Image	Note
TS22: intercostal muscle	Present		3P	Expression is present in the intercostal muscle fibers attached to the sternum.

Structure	Level	Pattern	Image	Note
TS22: skeletal muscle	Strong		FG37	There is strong expression in striated skeletal muscles.
TS22: brain ventricular layer	Present		FG37
TS22: spinal cord ventricular layer	Present		FG37
TS22: hindbrain	Present	Regionally restricted	FG37	There is expression in the rhombic lip and in neuronal precursors, presumably from the neuroepithelium lining the anterior edge of the fourth ventricle.
TS22: medulla oblongata	Present	Regionally restricted	FG37	There is expression in the area postrema.
TS22: cerebellum	Present		FG37
TS22: cerebral cortex ventricular layer	Present	Graded	FG37	There is a rostrocaudal expression gradient.
TS22: spinal cord	Present	Single cells	FG37	Expression is present in numerous small, scattered cells.
TS22: cartilage	Present		FG37
TS22: intestine	Present		FG37
TS22: liver	Present		FG37
TS22: lung	Present		FG37
TS22: metanephros	Present		FG37
TS22: pancreas	Present		FG37