Antibody:	Ymer antibody
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	Fixative was 3.7% formaldehyde. The expression level of Ymer protein in different mouse cochlear structures at different stages of development is summarized in Table 3.

Structure	Level	Pattern	Image	Note
TS22: inner ear	Strong	Spotted	5A	Strong punctate immunostaining was detected in the otic mesenchyme.
TS22: cochlear duct	Strong	Regionally restricted	5A	Expression is in the cells lining the lumen.
TS22: cochlear ganglion	Strong	Regionally restricted	5A	Expression is in the nerve fibers and in fibers invading the cochlear epithelium.

Structure	Level	Pattern	Image	Note
TS25: cochlea	Strong	Regionally restricted	5B	Expression is in the mesenchyme around the cochlear duct

Structure	Level	Pattern	Image	Note
TS27: organ of Corti	Present	Regionally restricted	5C	Expression is in the apical region of the developing pillar cells.
TS27: spiral limbus	Strong	Regionally restricted	5C	Expression is in the mesenchyme.
TS27: stria vascularis	Present	Regionally restricted	5C	Expression is in marginal cells.
TS27: cochlea	Present	Regionally restricted	5C	Expression is in the cytoplasm of the outer hair cells and in their innervating nerve fibers.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Strong	Regionally restricted	5D	Expression is through the entire length of the inner and outer pillar cells.
TS28: spiral ligament	Present		5D
TS28: spiral limbus	Present		5D
TS28: stria vascularis	Present		5D

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Strong	Regionally restricted	5E	Expression is through the entire length of the inner and outer pillar cells.
TS28: spiral ligament	Present		5E
TS28: spiral limbus	Present		5E
TS28: stria vascularis	Present		5E

Structure	Level	Pattern	Image	Note
TS28: stria vascularis	Strong	Regionally restricted	5F	Expression is in the marginal cells.
TS28: organ of Corti	Strong	Regionally restricted	5F	Expression is in the pillar cells and Deiter's cells.
TS28: spiral ligament	Weak		5F
TS28: spiral limbus	Weak		5F

Structure	Level	Pattern	Image	Note
TS28: stria vascularis	Strong	Regionally restricted	5G	Expression is in the marginal cells.
TS28: organ of Corti	Strong	Regionally restricted	5G	Expression is in the pillar cells and Deiter's cells.
TS28: spiral ligament	Weak		5G
TS28: spiral limbus	Weak		5G

Structure	Level	Pattern	Image	Note
TS28: stria vascularis	Strong	Regionally restricted	5H	Expression is in the marginal cells.
TS28: organ of Corti	Strong	Regionally restricted	5H	Expression is in the pillar cells and in the cell bodies and the processes of Deiter's cells.
TS28: spiral ligament	Weak		5H
TS28: spiral limbus	Weak		5H

Structure	Level	Pattern	Image	Note
TS28: stria vascularis	Strong	Regionally restricted	5I	Expression is in the marginal cells.
TS28: organ of Corti	Strong	Regionally restricted	5I	Expression is in the pillar cells and in the cell bodies and the processes of Deiter's cells.
TS28: spiral ligament	Weak		5I
TS28: spiral limbus	Weak		5I

Structure	Level	Pattern	Image	Note
TS28: stria vascularis	Present		5J
TS28: organ of Corti	Strong	Regionally restricted	5J	Expression is in the pillar cells.

Structure	Level	Pattern	Image	Note
TS28: stria vascularis	Present		5K
TS28: organ of Corti	Strong	Regionally restricted	5K	Expression is in the pillar cells.

Structure	Level	Pattern	Image	Note
TS25: inner ear vestibular component	Strong	Regionally restricted	6A	Expression is in the mesenchyme and in some cells of the vestibular epithelium.
TS25: vestibulocochlear nerve vestibular component	Present	Regionally restricted	6A	Expression is in the nerve fibers innervating the sensory epithelia.

Structure	Level	Pattern	Image	Note
TS25: utricle	Present	Regionally restricted	6B	Expression is in the mesenchyme and in some cells of the vestibular epithelium.
TS25: vestibulocochlear nerve vestibular component	Present	Regionally restricted	6B	Expression is in the nerve fibers innervating the sensory epithelia.

Structure	Level	Pattern	Image	Note
TS27: inner ear vestibular component	Strong	Regionally restricted	6C	Expression is in the mesenchyme and epithelium.
TS27: vestibulocochlear nerve vestibular component	Present	Regionally restricted	6C	Expression is in the nerve fibers innervating the sensory epithelia.

Structure	Level	Pattern	Image	Note
TS27: saccule	Present	Regionally restricted	6D	Expression is in the mesenchyme and epithelium.

Structure	Level	Pattern	Image	Note
TS28: inner ear vestibular component	Present	Regionally restricted	6E	Expression is in the mesenchyme and in the apical cytoplasm of the epithelial cells.

Structure	Level	Pattern	Image	Note
TS28: macula of utricle	Present	Regionally restricted	6F	Expression is in the apical cytoplasm of the epithelial cells.
TS28: utricle epithelium	Present	Regionally restricted	6F	Expression is in the apical cytoplasm of the epithelial cells.