Antibody:	6799
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Although the antibody used in the assay recognizes all isoforms on Westerns, it does not bind to the GAD65 isoform in immunohistochemical studies under the conditions used in this study. Based on other experiments presented in this paper, most of the expression seen during embryonic stages is due to GAD25 and GAD44 immunoreactivity; only adult GAD67 is expressed beyond P7.

Structure	Level	Pattern	Image	Note
TS15: lens placode	Present		3A

Structure	Level	Pattern	Image	Note
TS17: lens pit	Present	Uniform	3B

Structure	Level	Pattern	Image	Note
TS19: lens vesicle	Present	Uniform	3C

Structure	Level	Pattern	Image	Note
TS20: lens vesicle anterior epithelium	Strong		3D
TS20: lens vesicle posterior epithelium	Strong		3D	Expression is observed at the apical and basal cell poles of the elongating lens fibers.

Structure	Level	Pattern	Image	Note
TS22: lens fiber	Present		3E	Expression is present in both primary and secondary fiber cells.

Structure	Level	Pattern	Image	Note
TS23: lens fiber	Present		3F	Expression is present in both primary and secondary fiber cells. It is observed at the apical and basal cell poles of the elongating lens fibers.

Structure	Level	Pattern	Image	Note
TS24: lens fiber	Present		3G	Expression is present in both primary and secondary fiber cells.

Structure	Level	Pattern	Image	Note
TS25: lens fiber	Present		3H	Expression is present in both primary and secondary fiber cells.

Structure	Level	Pattern	Image	Note
TS27: lens fiber	Moderate		3I

Structure	Level	Pattern	Image	Note
TS28: lens fiber	Present		3J	Expression is present in elongating secondary fibers at the equatorial region.

Structure	Level	Pattern	Image	Note
TS28: lens fiber	Present		3K	Expression is present in elongating secondary fibers at the equatorial region.

Structure	Level	Pattern	Image	Note
TS28: lens fiber	Present	Regionally restricted	3L	Expression is present in elongating secondary fibers at the equatorial region. There is no expression in the lens core. Higher magnification was shown in the inset.

Structure	Level	Pattern	Image	Note
TS24: lens fiber	Present		4D , 4A	Expression is highly enriched in the apical and basal tips of elongating primary fibers.
TS24: lens capsule	Present		4A , 4D
TS24: lens epithelium	Present		4A , 4D	Expression is present at the fiber/lens epithelium interface.

Structure	Level	Pattern	Image	Note
TS24: lens fiber	Present		4B	Expression is highly enriched in the apical and basal tips of elongating primary fibers.
TS24: lens capsule	Present		4B
TS24: lens epithelium	Present		4B	Expression is present at the fiber/lens epithelium interface.

Structure	Level	Pattern	Image	Note
TS24: lens	Present		4C , 4E	The strongest signal is at the anterior and posterior lens poles, at sites of contact between (primary) lens fibers of opposite sides during formation of the prospective lens sutures.
TS24: lens fiber	Present		4E , 4C	Expression is highly enriched in the apical and basal tips of elongating primary fibers.
TS24: lens epithelium	Present	Regionally restricted	4E , 4C	Expression is present in the central parts of the lens epithelium. It disappears in the adjacent germinative epithelium.
TS24: cornea	Present	Regionally restricted	4E , 4C	Expression disappears gradually toward the flanking regions.