Gene Expression Data
Assay Details
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Assay
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Reference:
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J:127151
Kwakowsky A, et al., GAD isoforms exhibit distinct spatiotemporal expression patterns in the developing mouse lens: Correlation with Dlx2 and Dlx5. Dev Dyn. 2007 Dec;236(12):3532-44
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:3766399
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Gene symbol:
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Gad1
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Gene name:
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glutamate decarboxylase 1
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Probe:
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EGAD-pA, EGAD-pB
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Visualized with:
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Ethidium bromide
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Assay notes:
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The primer set used for this assay amplifies products from the 25 kDa and 44 kDa isoforms. RT-PCR reactions were performed in triplicate using RNA from two independent tissue preparations.
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Results
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| E14.5
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TS22: lens
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Strong
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3 µg; total RNA |
C57BL/6 |
| Not Specified |
| E15.5
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TS23: lens
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Strong
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3 µg; total RNA |
C57BL/6 |
| Not Specified |
| E16.5
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TS24: lens
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Strong
(a)
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3 µg; total RNA |
C57BL/6 |
| Not Specified |
| E17.5
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TS25: lens
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Present
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3 µg; total RNA |
C57BL/6 |
| Not Specified |
| P0
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TS27: lens
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Present
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3 µg; total RNA |
C57BL/6 |
| Not Specified |
| P7
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TS28: lens
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Trace
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3 µg; total RNA |
C57BL/6 |
| Not Specified |
| P14
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TS28: lens
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Trace
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3 µg; total RNA |
C57BL/6 |
| Not Specified |
| P30
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TS28: lens
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Trace
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3 µg; total RNA |
C57BL/6 |
| Not Specified |
| Control |
| P adult
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TS28: brain
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Present
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3 µg; total RNA |
C57BL/6 |
| Not Specified |
Notes:
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Analysis Tools