Plxnb1tm1Matl
Targeted Allele Detail
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| Symbol: |
Plxnb1tm1Matl |
| Name: |
plexin B1; targeted mutation 1, Marc Tessier-Lavigne |
| MGI ID: |
MGI:3765917 |
| Synonyms: |
Plxnb1-, Plxnb1-lacZ |
| Gene: |
Plxnb1 Location: Chr9:108924457-108948985 bp, + strand Genetic Position: Chr9, 59.63 cM
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| Alliance: |
Plxnb1tm1Matl page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:139221
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| Parent Cell Line: |
E14TG2a (ES Cell)
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| Strain of Origin: |
129P2/OlaHsd
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| Allele Type: |
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Targeted (Null/knockout, Reporter) |
| Mutation: |
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Insertion
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Mutation details: Targeted mutagenesis using a promoterless gene trap vector ("targeted trapping") was used to insert a 9.4-kb placental alkaline phosphatase (PLAP) secretory cassette into intron 21. This results in transcription of a fusion mRNA joining upstream exons of the endogenous gene to the elements of the cassette, comprising a splice acceptor and a transmembrane domain coding sequence followed by the betageo (lacZ/neomycin resistance fusion) cDNA, an internal ribosomal entry site (IRES), and the human PLAP sequence with a polyadenylation signal. Two proteins are translated from this bicistronic transcript: a PLXNB1/Betageo fusion protein that is sequestered in an intracellular compartment of the neuronal cell body; and PLAP, which is expressed on the axonal surface. (Roland Freidel, data submission). Western blot of E11.5 whole embryo lysates confirmed the absence of protein in homozygous mutants.
(J:228347)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Original: |
J:121225 Friedel RH, et al., Gene targeting using a promoterless gene trap vector ('targeted trapping') is an efficient method to mutate a large fraction of genes. Proc Natl Acad Sci U S A. 2005 Sep 13;102(37):13188-93 |
| All: |
8 reference(s) |
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Analysis Tools
