Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Trpc1tm1Lbi
Name:	transient receptor potential cation channel, subfamily C, member 1; targeted mutation 1, Lutz Birnbaumer
MGI ID:	MGI:3764882
Gene:	Trpc1  Location: Chr9:95587135-95632428 bp, - strand  Genetic Position: Chr9, 50.2 cM, cytoband E4
Alliance:	Trpc1tm1Lbi page

Germline Transmission:	Earliest citation of germline transmission: J:127788
Parent Cell Line:	CCE/EK.CCE (ES Cell)
Strain of Origin:	129S/SvEv-Gpi1c

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: An EcoRI site in exon 8 was converted to a stop codon and a PGK-neo selection cassette replaced the terminal part of exon 8 along with intron 8. This resulted in loss of the coding region for amino acids 584 - 670 encoding transmembrane segments 4 and 5 and a small part of the putative pore domain. RT-PCR analysis detected a shorter transcript in which all of exon 8 was skipped resulting in a frameshift and stop codon at position 2 of exon 9. Western blot analysis confirmed absence of expression in the submandibular glands of homozygous mice. (J:127118, J:127788)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

3 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Trpc1 Mutation:	39 strains or lines available

Original:	J:127788 Dietrich A, et al., Pressure-induced and store-operated cation influx in vascular smooth muscle cells is independent of TRPC1. Pflugers Arch. 2007 Dec;455(3):465-77
All:	57 reference(s)