Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:85300
Helmbacher F, et al., Met signaling is required for recruitment of motor neurons to PEA3-positive motor pools. Neuron. 2003 Aug 28;39(5):767-77
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:3763258
|
|
Gene symbol:
|
Pou3f1
|
|
Gene name:
|
POU domain, class 3, transcription factor 1
|
|
Probe:
|
Scip probe1
|
|
Probe preparation:
|
labelled with digoxigenin RNA
|
|
|
Results
|
Specimen
S1C:
embryonic day 12.5
(more  )
Note: The specimen was a flat-mounted spinal cord.
Specimen
S1C:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
The specimen was a flat-mounted spinal cord.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
S1C
|
Expression was detected in a subpopulation of lateral motor column motor neurons.
|
Specimen
S1D:
embryonic day 12.5; Mettm1Cpo/ Mettm1Cpo
(more )
Note: The specimen was a flat-mounted spinal cord.
Specimen
S1D:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
|
|
Mutant Allele(s):
|
Mettm1Cpo/Mettm1Cpo
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
The specimen was a flat-mounted spinal cord.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
|
S1D
|
No changes in expression levels were detected in the mutant.
|
Specimen
S1I:
embryonic day 14.5
(more )
Note: The specimen was a flat-mounted spinal cord.
Specimen
S1I:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
The specimen was a flat-mounted spinal cord.
|
Specimen
S1J:
embryonic day 14.5; Mettm1Cpo/ Mettm1Cpo
(more )
Note: The specimen was a flat-mounted spinal cord.
Specimen
S1J:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
|
|
Mutant Allele(s):
|
Mettm1Cpo/Mettm1Cpo
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
The specimen was a flat-mounted spinal cord.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord |
Trace |
|
S1J
|
Expression was almost completely absent in the mutant spinal cord.
|
|
Analysis Tools