Gene Expression Data
Assay Details
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Assay
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Reference:
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J:85300
Helmbacher F, et al., Met signaling is required for recruitment of motor neurons to PEA3-positive motor pools. Neuron. 2003 Aug 28;39(5):767-77
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3763234
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Gene symbol:
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Etv4
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Gene name:
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ets variant 4
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Probe:
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Pea3 probe1
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Probe preparation:
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labelled with digoxigenin RNA
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Results
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Specimen
1A:
embryonic day 11.5
(more  )
Note: 49 somites. The specimen was a flat-mounted spinal cord.
Specimen
1A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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49 somites. The specimen was a flat-mounted spinal cord.
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Specimen
1B:
embryonic day 11.5; Mettm1Cpo/ Mettm1Cpo
(more )
Note: 49 somites. The specimen was a flat-mounted spinal cord.
Specimen
1B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Mettm1Cpo/Mettm1Cpo
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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49 somites. The specimen was a flat-mounted spinal cord.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
Present |
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1B
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Mutant spinal cord showed a significant reduction of expression.
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Specimen
S1A:
embryonic day 12.5
(more )
Note: The specimen was a flat-mounted spinal cord.
Specimen
S1A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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The specimen was a flat-mounted spinal cord.
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Specimen
S1B:
embryonic day 12.5; Mettm1Cpo/ Mettm1Cpo
(more )
Note: The specimen was a flat-mounted spinal cord.
Specimen
S1B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Mettm1Cpo/Mettm1Cpo
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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The specimen was a flat-mounted spinal cord.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
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S1B
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The anteroposterior extent of expression was reduced in mutant spinal cord.
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Specimen
S1G:
embryonic day 14.5
(more )
Note: The specimen was a flat-mounted spinal cord.
Specimen
S1G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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The specimen was a flat-mounted spinal cord.
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Specimen
S1H:
embryonic day 14.5; Mettm1Cpo/ Mettm1Cpo
(more )
Note: The specimen was a flat-mounted spinal cord.
Specimen
S1H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Mettm1Cpo/Mettm1Cpo
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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The specimen was a flat-mounted spinal cord.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord |
Trace |
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S1H
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Expression was almost completely absent in mutant spinal cord.
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