Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(Thy1-Brainbow1.0)HLich
Name:	transgene insertion H, Jeff W Lichtman
MGI ID:	MGI:3761285
Synonyms:	Tg(Thy1-tdimer2(12),-mYFP,-mCerulean)H10Lich, Thy1-Brainbow-1.0 line H
Transgene:	Tg(Thy1-Brainbow1.0)HLich  Location: unknown
Alliance:	Tg(Thy1-Brainbow1.0)HLich page

Strain of Origin:

C57BL/6 x CBA

Transgene Type:    Transgenic (Reporter) Mutation:    Insertion   Mutation details: The Thy1-Brainbow 1.0 transgene was designed with the mouse Thy1 regulatory elements surrounding three fluorescent protein (red (RFP), enhanced yellow (EYFP), or cyan (CFP)) sequences uniquely flanked with pairs of incompatible Lox sites. Specifically, this Brainbow 1.0 coding region contained (from 5'to 3') a Lox2272 site, LoxP site, tdimer2(12) RFP (with polyA sequence), Lox2272 site, monomeric EYFP (mYFPA206K; with membrane tethering palmitoylation sequence and polyA sequence), LoxP site, mCerulean CFP (with membrane tethering palmitoylation sequence and 5'FRT site and polyA sequence). This transgene was microinjected into C57BL/6JxCBA hybrid oocytes. The tdimer2(12) RFP is a non-oligomerizing DsRed variant with a 12 residue linker fusing two copies of the protein (tandem dimer). The monomeric EYFP (mYFPA206K) has an amino acid substitution replacing a hydrophobic region with a positively charged residue designed to prevent dimerization. The monomeric Cerulean (mCerulean) is a variant of ECFP (ECFPS72A/Y145A/H148D/A206K) with amino acid substitutions designed to improve spectral properties and prevent dimerization. (J:125961)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:125961 Livet J, et al., Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system. Nature. 2007 Nov 1;450(7166):56-62
All:	6 reference(s)