Probe:	Hox11 probe2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch ectoderm	Strong		4A	Expression is present in the surface ectoderm.
TS15: 2nd branchial arch ectoderm	Strong		4A	Expression is present in the surface ectoderm.
TS15: 1st branchial arch mesenchyme	Strong	Regionally restricted	4A	Expression is present in the central mesenchyme.
TS15: 2nd branchial arch mesenchyme	Strong	Regionally restricted	4A	Expression is present in the central mesenchyme.
TS15: pharynx	Present		4A	There is a diffuse band extending dorsally in the region of the presumptive pharynx.

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Strong		4B	There is strong expression in the peripheral margins.
TS17: facial ganglion	Strong		4B	There is strong expression in the peripheral margins.
TS17: trigeminal ganglion	Strong		4B	There is strong expression in the peripheral margins.
TS17: 1st branchial arch ectoderm	Present		4B	Expression is present in the surface ectoderm.

Structure	Level	Pattern	Image	Note
TS17: glossopharyngeal ganglion	Present		4C
TS17: future spinal cord	Present	Non-Uniform	4C	There are discontinuous regions of staining.
TS17: 1st branchial arch ectoderm	Present		4C	Expression is present in the surface ectoderm.
TS17: 2nd branchial arch ectoderm	Present		4C	Expression is present in the surface ectoderm.
TS17: 1st branchial arch mesenchyme	Present		4C	Expression is present in the central mesenchyme.
TS17: 2nd branchial arch mesenchyme	Present		4C	Expression is present in the central mesenchyme.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch ectoderm	Present		4D	Expression is present in the surface ectoderm.
TS17: 2nd branchial arch ectoderm	Present		4D	Expression is present in the surface ectoderm.
TS17: 3rd branchial arch ectoderm	Present		4D	Expression is present in the surface ectoderm.
TS17: 4th branchial arch ectoderm	Present		4D	Expression is present in the surface ectoderm.
TS17: 1st branchial arch mesenchyme	Present		4D	Expression is present in the central mesenchyme.
TS17: 2nd branchial arch mesenchyme	Present		4D	Expression is present in the central mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: medulla oblongata	Strong	Regionally restricted	5A	Expression is present in two bands on the dorsal and ventral aspects of the curvature of the pons-medulla of the hindbrain. This expression is restricted to the midline of the embryo and is absent from lateral regions of the hindbrain.
TS20: pons	Strong	Regionally restricted	5A	Expression is present in two bands on the dorsal and ventral aspects of the curvature of the pons-medulla of the hindbrain. This expression is restricted to the midline of the embryo and is absent from lateral regions of the hindbrain.
TS20: trigeminal ganglion	Strong	Uniform	5A
TS20: pancreas	Strong		5A

Structure	Level	Pattern	Image	Note
TS20: spinal cord mantle layer	Present	Regionally restricted	5B	Expression is present in diffuse, centrally located band extending from the base of the hindbrain to the lumbar region.
TS20: tongue epithelium	Present		5B
TS20: mandibular process	Present	Regionally restricted	5B	Expression is present in the surface epithelium.
TS20: tongue mesenchyme	Present	Regionally restricted	5B	Expression is present in the lingual mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: spinal cord mantle layer	Present			Expression is present in a diffuse, centrally located band extending to the caudal-most end of the spinal cord.

Structure	Level	Pattern	Image	Note
TS21: pinna	Present		5C	Expression is present in a discrete band lining the internal margin of the pinna, extending along an arc towards the vestibulocochlear ganglion.

Structure	Level	Pattern	Image	Note
TS20: tongue epithelium	Present	Regionally restricted	6A/B	Expression is present in the dorsal aspect and distal region of the lingual surface epithelium.
TS20: tongue mesenchyme	Present	Graded	6A/B	There is a distal to proximal gradient of expression evident in the tongue mesenchyme.
TS20: mandibular process	Present	Regionally restricted	6A/B	Expression in the mandibular epithelium extends over the dorsal aspect and further concentrates in regions of its thickening and invagination at sites of developing tooth primordia.

Structure	Level	Pattern	Image	Note
TS21: tongue epithelium	Present		6C/D
TS21: tongue mesenchyme	Present		6C/D
TS21: lower jaw epithelium	Present		6C/D	Expression is present in the mandibular epithelium and clearly marks the tooth bud.
TS21: lower jaw tooth	Present		6C/D	Expression is present in the mandibular epithelium and clearly marks the tooth bud.
TS21: lower jaw mesenchyme	Present		6C/D	Expression is present in tracts of condensing mandibular mesenchyme, thought to represent presumptive muscle.

Structure	Level	Pattern	Image	Note
TS21: trigeminal ganglion	Strong		7A
TS21: pons	Present	Regionally restricted	7A	Expression is present in the caudal region.
TS21: medulla oblongata	Present	Regionally restricted	7A	Expression is present in the rostral region.
TS21: vestibulo-cochlear ganglion	Strong		7A	Although expression is clearly present in the vestibulocochlear ganglia, sections failed to demonstrate whether there is also expression in the facial ganglion.

Structure	Level	Pattern	Image	Note
TS21: tongue extrinsic pre-muscle mass	Present		7B	Expression is present in the intrinsic and extrinsic muscle tracts of the tongue.
TS21: tongue muscle	Present		7B	Expression is present in the intrinsic and extrinsic muscle tracts of the tongue.
TS21: submandibular gland primordium	Present		7B

Structure	Level	Pattern	Image	Note
TS21: tongue extrinsic pre-muscle mass	Present		7C	Expression is present in the intrinsic and extrinsic muscle tracts of the tongue.
TS21: tongue muscle	Present		7C	Expression is present in the intrinsic and extrinsic muscle tracts of the tongue.
TS21: pinna	Present		7C	Expression is present in a discrete band lining the internal margin of the pinna, extending along an arc towards the vestibulocochlear ganglion.

Structure	Level	Pattern	Image	Note
TS21: tongue extrinsic pre-muscle mass	Present		7D	Expression is present in the intrinsic and extrinsic muscle tracts of the tongue.
TS21: tongue muscle	Present		7D	Expression is present in the intrinsic and extrinsic muscle tracts of the tongue.
TS21: external auditory meatus	Present	Regionally restricted	7D	Expression is present in the lining.

Structure	Level	Pattern	Image	Note
TS23: tongue epithelium	Present		8A/B
TS23: tongue mesenchyme	Absent		8A/B
TS23: tooth	Strong		8A/B	There is strong hybridization in developing tooth primorida.
TS23: submandibular gland primordium	Strong		8A/B	Expression appears to be evenly distributed throughout the lobules.
TS23: cartilage	Present		8A/B	There is expression in condensing mesenchyme, presumably cartilage, of the ribs and vertebrae.
TS23: trigeminal ganglion	Absent		8A/B
TS23: vestibulo-cochlear ganglion	Absent		8A/B

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Absent
TS23: pons	Present	Regionally restricted		Expression is present in the caudal region.
TS23: medulla oblongata	Present	Regionally restricted		Expression is present in the rostral region.
TS23: pancreas	Absent

Structure	Level	Pattern	Image	Note
TS24: lower jaw tooth	Present			Expression is present in developing tooth primordia.
TS24: submandibular gland	Present

Structure	Level	Pattern	Image	Note
TS28: tongue papilla epithelium	Present		9B/C , 9A	Expression is localized to the keratinized epithelium of papillae.

Structure	Level	Pattern	Image	Note
TS20: pancreas	Strong	Non-Uniform	10B	There is strong expression in the pancreatic primorida. There are focal areas of weak or no expression corresponding to the columnar epithelium lining the interlobular ducts.

Structure	Level	Pattern	Image	Note
TS20: pancreatic duct	Strong			There is strong expression in the pancreatic duct.

Structure	Level	Pattern	Image	Note
TS22: pancreas	Present

Structure	Level	Pattern	Image	Note
TS27: thymus	Absent