Hap1 Western blot Gene Expression Assay - GXD

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Gene Expression Data
Assay Details

Assay

Reference:	J:76174 Chan EY, et al., Targeted disruption of Huntingtin-associated protein-1 (Hap1) results in postnatal death due to depressed feeding behavior. Hum Mol Genet. 2002 Apr 15;11(8):945-59
Assay type:	Western blot
MGI Accession ID:	MGI:3758029
Gene symbol:	Hap1
Gene name:	huntingtin-associated protein 1

Antibody:	1B6
Detection system:	Secondary antibody coupled to horseradish peroxidase

Results

Image: 2A Hap-1A & Hap-1B

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	100 kDa (a)	100 kDa (a)	Genetic Background	Mutant Allele(s)	Sex	Note
Lane 1	P1	TS27: brain	Present	Present	Not Specified		Not Specified	Brain sample excluded the olfactory bulb and brainstem.
Lane 2	P1	TS27: brain	Present	Present	Not Specified		Not Specified	Brain sample excluded the olfactory bulb and brainstem.
Lane 3	P1	TS27: brain	Present	Present	involves: 129S1/Sv * 129X1/SvJ * ICR	Hap1^tm1Hay/Hap1⁺	Not Specified	Brain sample excluded the olfactory bulb and brainstem.
Lane 4	P1	TS27: brain	Present	Present	involves: 129S1/Sv * 129X1/SvJ * ICR	Hap1^tm1Hay/Hap1⁺	Not Specified	Brain sample excluded the olfactory bulb and brainstem.
Lane 5	P1	TS27: brain	Absent	Absent	involves: 129S1/Sv * 129X1/SvJ * ICR	Hap1^tm1Hay/Hap1^tm1Hay	Not Specified	Brain sample excluded the olfactory bulb and brainstem.
Lane 6	P1	TS27: brain	Absent	Absent	involves: 129S1/Sv * 129X1/SvJ * ICR	Hap1^tm1Hay/Hap1^tm1Hay	Not Specified	Brain sample excluded the olfactory bulb and brainstem.

Notes:
(a) The antibody detected both Hap-1A and Hap-1B at approximately 100kDa. Equivalent high levels of Hap-1A and Hap-1B were observed in Hap1+/+ and +/- mice.

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