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Tg(Ddx4-cre)1Dcas
Transgene Detail
Summary
Symbol: Tg(Ddx4-cre)1Dcas
Name: transgene insertion 1, Diego H Castrillon
MGI ID: MGI:3757577
Synonyms: Mvh-Cre, Vasa-Cre
Transgene: Tg(Ddx4-cre)1Dcas  Location: Chr18:85714737-86812993 bp  Genetic Position: Chr18, Syntenic
Alliance: Tg(Ddx4-cre)1Dcas page
Transgene
origin
Strain of Origin:  FVB
Transgene
description
Transgene Type:    Transgenic (Null/knockout, Recombinase)
Mutations:    Insertion, Intergenic deletion, Intragenic deletion
  Tg(Ddx4-cre)1Dcas involves 2 genes/genome features (Neto1, Cbln2) View all
 
Tg(Ddx4-cre)1Dcas expression driven by 1 gene
 
Mutation detailsA 5.6kb fragment of the Ddx4 promoter was ligated to the cre open reading frame with an engineered nuclear localization signal. Eight transgenic lines were created but only two possessed expressing in the gonads as early as E15. When the cre transgene is maternally expressed, all progeny can show ubiquitous recombination even if the cre is not inherited, likely due to perdurance of cre protein in the egg. The transgene integrated into chromosome 18 causing a 1098 Kb deletion and deleting the genes Neto1 and Cbln2. The deletion results in a functional knock-out of both genes in homozygous mice. Founder line 1 has a copy number of greater than 20. (J:125051)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Ddx4 (species not specified)
Summary of all recombinase alleles driven by Ddx4.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Expression
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
References
Original:  J:125051 Gallardo T, et al., Generation of a germ cell-specific mouse transgenic Cre line, Vasa-Cre. Genesis. 2007 Jun;45(6):413-7
All:  172 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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last database update
03/12/2024
MGI 6.23
The Jackson Laboratory