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Gene Expression Data
Assay Details
Assay
Reference: J:99722 Verbsky J, et al., Disruption of the mouse inositol 1,3,4,5,6-pentakisphosphate 2-kinase gene, associated lethality, and tissue distribution of 2-kinase expression. Proc Natl Acad Sci U S A. 2005 Jun 14;102(24):8448-53
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:3722915
Gene symbol: Ippk
Gene name: inositol 1,3,4,5,6-pentakisphosphate 2-kinase
Reporter: lacZ
Direct Detection
Assay notes: Fixative is 0.2% glutaraldehyde/0.1 M MgCl2/5 mM EGTA in PBS.
Results
Specimen 3A: postnatal adult; IppkGt(XA232)Byg/Ippk+ (more )
Structure Level Pattern Image Note
TS28: dentate gyrus Present 3A
TS28: hippocampus CA1 Present 3A
TS28: hippocampus CA2 Present 3A
TS28: hippocampus CA3 Present 3A

Specimen 3B: postnatal adult; IppkGt(XA232)Byg/Ippk+ (more )
Structure Level Pattern Image Note
TS28: neocortex layer Present Regionally restricted 3B Expression is in the inner layers of the cerebral cortex.

Specimen 3C: postnatal adult; IppkGt(XA232)Byg/Ippk+ (more )
Structure Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Present 3C

Specimen 3D: postnatal adult; IppkGt(XA232)Byg/Ippk+ (more )
Structure Level Pattern Image Note
TS28: heart Present Regionally restricted 3D Punctate expression in cardiomyocytes but expression is absent in interstitial cells.
TS28: blood vessel Absent 3D
TS28: heart valve Absent 3D

Specimen Not shown: postnatal adult; IppkGt(XA232)Byg/Ippk+ (more )
Structure Level Pattern Image Note
TS28: testis Present Diffuse

Specimen 3E: embryonic day 9.0; IppkGt(XA232)Byg/Ippk+ (more )
Structure Level Pattern Image Note
TS14: neural tube Strong 3E
TS14: notochord Strong 3E
TS14: somite Strong 3E

Specimen 3F: embryonic day 9.0; IppkGt(XA232)Byg/Ippk+ (more )
Structure Level Pattern Image Note
TS14: neural tube lateral wall Present Regionally restricted 3F Expression is in the ventricular zone and in migrating neuroblasts.
TS14: somite Strong Regionally restricted 3F Expression is in specific regions.
TS14: notochord Strong 3F

Specimen 3G: embryonic day 9.0; IppkGt(XA232)Byg/Ippk+ (more )
Structure Level Pattern Image Note
TS14: trunk myotome Strong 3G
TS14: neural tube lateral wall Strong Regionally restricted 3G Expression is in the ventricular zone and in migrating neuroblasts.

Specimen 3H: embryonic day 9.0; IppkGt(XA232)Byg/Ippk+ (more )
Structure Level Pattern Image Note
TS14: future brain Strong Regionally restricted 3H Expression is in the ventricular zone and in migrating neuroblasts.

Specimen 3I: embryonic day 9.0; IppkGt(XA232)Byg/Ippk+ (more )
Structure Level Pattern Image Note
TS14: yolk sac Present Regionally restricted 3I Expression is in epithelial and endothelial layers and is absent from blood islands.

Specimen 3J: embryonic day 9.0; IppkGt(XA232)Byg/Ippk+ (more )
Structure Level Pattern Image Note
TS14: yolk sac Present Regionally restricted 3J Expression is in epithelial and endothelial layers and is absent from blood islands.

Specimen 3K: embryonic day 9.0; IppkGt(XA232)Byg/Ippk+ (more )
Structure Level Pattern Image Note
TS14: heart Present Regionally restricted 3K Expression is in the ventricular chamber.
TS14: common atrial chamber Present 3K
TS14: primitive ventricle cardiac muscle Present 3K The inset shows a magnified view of this.
TS14: primitive ventricle endocardial tube Present 3K The inset shows a magnified view of this.

Specimen Not shown: embryonic day 9.0; IppkGt(XA232)Byg/Ippk+ (more )
Structure Level Pattern Image Note
TS14: branchial arch Present
TS14: cardinal vein Present
TS14: dorsal aorta Present
TS14: gut Present


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/16/2024
MGI 6.23
The Jackson Laboratory