Gene Expression Data
Assay Details
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Assay
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Reference:
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J:93172
Resendes BL, et al., Isolation from cochlea of a novel human intronless gene with predominant fetal expression. J Assoc Res Otolaryngol. 2004 Jun;5(2):185-202
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3722428
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Gene symbol:
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Kctd12
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Gene name:
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potassium channel tetramerisation domain containing 12
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Antibody:
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anti-PFET1
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Detection system:
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Secondary antibody coupled to biotin
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Assay notes:
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Authors report that specimens were fixed in formalin gluraraldehyde.
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Results
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Specimen
8B:
postnatal adult
(more  )
Specimen
8B:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spiral ligament |
Strong |
Regionally restricted |
8B
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Dominant staining is seen in type I fibrocytes (T1) in the spiral ligament.
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| TS28: cochlear duct |
Present |
Regionally restricted |
8B
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Immunostaining also localized in types IV (T4) and type V (T5) fibrocytes, Deiters' cells (DT), interdental cells (IDC), limbal fibrocytes (LF) and supralimbal fibrocytes.
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| TS28: cochlear ganglion |
Present |
Regionally restricted |
8B
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Expression in spiral ganglion neurons.
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Specimen
not shown:
postnatal adult
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: brain |
Present |
Regionally restricted |
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Immunostained neurons included Scarpa's ganglion cells, and Purkinje cells in sections containing brain tissues.
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| TS28: cochlea |
Present |
Regionally restricted |
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Immunostained neurons included Scarpa's ganglion cells, and Purkinje cells in sections containing brain tissues.
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Specimen
10A:
postnatal adult
(more )
Specimen
10A:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cochlear duct |
Present |
Regionally restricted |
10A
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Expression in Delters' cells (DT), Hensen cells (HC), Iinner pillar cells (IPC) and outer pillar cells (OPC).
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Specimen
10B:
postnatal adult
(more )
Specimen
10B:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cochlear duct |
Present |
Regionally restricted |
10B
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Expression in interdental cells (IDC), inner sulcus cells (ISC), limbal fibrocytes LF).
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| TS28: cochlear ganglion |
Present |
Regionally restricted |
10B
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Expression in spiral ganglion neurons.
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Specimen
11G:
postnatal adult
(more )
Specimen
11G:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: macula of saccule |
Present |
Regionally restricted |
11G
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Staining of type I hair cells in the hair cell cytoplasm. Ciliary bundle extending from the cuticular plate was unstained.
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| TS28: saccule epithelium |
Present |
Regionally restricted |
11G
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Staining of type I hair cells in the hair cell cytoplasm. Ciliary bundle extending from the cuticular plate was unstained.
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Specimen
11H:
postnatal adult
(more )
Specimen
11H:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: inner ear vestibular component |
Present |
Regionally restricted |
11H
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In the lateral ampulla, staining in luminal and abluminal cells, apical staining of type I hair cells at the cuticular plate, and staining in connective fibrocytes underneath sensory epithelium.
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