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Tg(Gad1-EGFP)3Gfng
Transgene Detail
Summary
Symbol: Tg(Gad1-EGFP)3Gfng
Name: transgene insertion 3, Guoping Feng
MGI ID: MGI:3721803
Synonyms: GAD67-GFP, GAD67-GFP BAC, GAD67-GFP (line 3)
Transgene: Tg(Gad1-EGFP)3Gfng  Location: unknown  
Alliance: Tg(Gad1-EGFP)3Gfng page
Transgene
origin
Strain of Origin:  (C57BL/6J x SJL)F1
Transgene
description
Transgene Type:    Transgenic (Reporter)
Mutation:    Insertion
 
Mutation detailsThe bacterial artificial chromosome (BAC) clone RP23-118N13 containing the mouse GAD67 gene (and 120 kb of upstream and 46 kb downstream sequence) was modified by insertion of an Enhanced Green Fluorescent Protein (EGFP) cDNA and polyadenylation sequence at the translation initiation site of the GAD67 gene. This GAD67-GFP BAC was microinjected into fertilized (C57BL/6J x SJL)F1 oocytes. Founder line 3 was established. Selective transgene expression is observed in newborn dentate granule cells of the adult hippocampus (subgranular zone of the dentate gyrus). EGFP is strongly expressed in the Purkinje cells of the cerebellum, periglomerular neurons of the olfactory bulb, and interneurons of the superior colliculus and brainstem with fluorescence observed in neurons (including axon terminals, dendrites and dendritic spines). Sparse EGFP labeling is reported in the thalamus, hypothalamus, cortex and striatum. (J:143860)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:143860 Feng G, Generation of a Gad1 reporter transgene. MGI Direct Data Submission. 2009;
All:  6 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory