Probe:	Emilin1 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS11: ectoplacental cone	Strong		2A
TS11: extraembryonic visceral endoderm	Strong		2A
TS11: chorion	Weak		2A
TS11: embryo	Absent		2A
TS11: primitive endoderm	Absent		2A

Structure	Level	Pattern	Image	Note
TS11: ectoplacental cone	Present	Graded	2B	Expression is in a gradient increasing from the embryonic to the maternal side: the most strongly stained cells include several layers of diploid cells and the layer of polyploid secondary trophoblast giant cells, which abuts the decidua.
TS11: extraembryonic visceral endoderm	Strong		2B
TS11: chorion	Weak		2B
TS11: embryo	Absent		2B
TS11: primitive endoderm	Absent		2B

Structure	Level	Pattern	Image	Note
TS9: ectoplacental cone	Present		2C
TS28: decidua	Present	Regionally restricted	2C	Expression begins at the periphery.

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted	2D	Exression is at the periphery and at the hilar side.

Structure	Level	Pattern	Image	Note
TS28: placenta	Strong	Regionally restricted	2E	Expression is in spongiotrophoblast and trophoblast giant cells surrounding the conceptus.

Structure	Level	Pattern	Image	Note
TS28: placenta	Strong	Regionally restricted	2F	Expression is in spongiotrophoblast and trophoblast giant cells surrounding the conceptus.

Structure	Level	Pattern	Image	Note
TS28: placenta	Strong	Regionally restricted	2G	Expression is in spongiotrophoblast and trophoblast giant cells surrounding the conceptus.

Structure	Level	Pattern	Image	Note
TS13: somite	Present		2H
TS13: embryo mesenchyme	Strong	Regionally restricted	2H	Expression is in perineural mesenchyme.

Structure	Level	Pattern	Image	Note
TS13: somite	Present		2I
TS13: extraembryonic component	Present	Regionally restricted	2I	Expression is in the extraembryonic mesoderm lining the yolk sac, amnios and blood islands.
TS13: allantois	Strong	Regionally restricted	2I
TS13: embryo mesenchyme	Strong	Regionally restricted	2I	Expression is in perineural mesenchyme.

Structure	Level	Pattern	Image	Note
TS15: yolk sac	Absent			Absent in the epithelial layer of yolk sac.

Structure	Level	Pattern	Image	Note
TS15: cardiac muscle tissue	Present		3A
TS15: endocardial lining	Present		3A

Structure	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	Present		3B
TS17: endocardial lining	Present		3B
TS17: liver	Present	Regionally restricted	3B	Expression is in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS19: atrium cardiac muscle	Weak		3C
TS19: ventricle cardiac muscle	Weak		3C
TS19: atrium endocardial lining	Present		3C
TS19: ventricle endocardial lining	Present		3C
TS19: liver	Present	Regionally restricted	3C	Expression is in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: atrium endocardial lining	Weak		3D
TS21: ventricle endocardial lining	Weak		3D
TS21: endocardial cushion	Strong		3D
TS21: pericardium	Present		3D

Structure	Level	Pattern	Image	Note
TS24: heart valve	Weak		3E
TS24: pericardium	Weak		3E

Structure	Level	Pattern	Image	Note
TS20: blood vessel	Present	Non-Uniform	3F	The intensity of staining varies with the anatomical location, strong expression in endothelia of blood vessels invading the central nervous system.

Structure	Level	Pattern	Image	Note
TS19: trunk sclerotome	Present		3G

Structure	Level	Pattern	Image	Note
TS19: branchial arch mesenchyme	Present		3H
TS19: limb mesenchyme	Present		3H
TS19: trunk sclerotome	Present		3H
TS19: central nervous system	Absent		3H
TS19: embryo ectoderm	Absent		3H
TS19: peripheral nervous system	Absent		3H

Structure	Level	Pattern	Image	Note
TS21: skeleton	Present	Regionally restricted	3I	Expression decreases as differentiation proceeds and becomes restricted to specific sites.
TS21: lung mesenchyme	Present		3I
TS21: mesenchyme of metanephros	Present		3I
TS21: liver	Present	Regionally restricted	3I	Expression is in the capsule.
TS21: gland	Present	Regionally restricted	3I	Expression is in the mesenchyme of endocrine glands.
TS21: axial skeleton	Present	Regionally restricted	3I	Expression is in intervertebral disks.
TS21: vertebral cartilage condensation	Present	Regionally restricted	3I	Expression is in the posterior part of vertebral cartilage.
TS21: embryo mesenchyme	Present	Regionally restricted	3I	Expression is in subepidermal mesenchyme.
TS21: vibrissa	Present		3I
TS21: central nervous system	Absent		3I
TS21: epidermis	Absent		3I
TS21: peripheral nervous system	Absent		3I

Structure	Level	Pattern	Image	Note
TS21: skeleton	Present	Regionally restricted	3J	Expression decreases as differentiation proceeds and becomes restricted to specific sites.
TS21: blood vessel	Present		3J
TS21: genital tubercle	Present		3J
TS21: embryo	Present		3J	Expression is in the connective tissue of interstitium and capsule.
TS21: liver	Present	Regionally restricted	3J	Expression is in the capsule.
TS21: axial skeleton	Present	Regionally restricted	3J	Expression is in intervertebral disks.
TS21: vertebral cartilage condensation	Present	Regionally restricted	3J	Expression is in the posterior part of vertebral cartilage.
TS21: embryo mesenchyme	Present	Regionally restricted	3J	Expression is in subepidermal mesenchyme.
TS21: vibrissa	Present		3J
TS21: central nervous system	Absent		3J
TS21: epidermis	Absent		3J
TS21: peripheral nervous system	Absent		3J

Structure	Level	Pattern	Image	Note
TS22: skeletal muscle	Present		3K	Expression is in connective and muscle tissues.
TS22: embryo	Strong	Regionally restricted	3K	Expression is in tendons.

Structure	Level	Pattern	Image	Note
TS24: metanephros	Present	Regionally restricted	3L	Expression is in connective tissue of the cortex and underling the pelvis epithelium.
TS24: intestine	Present	Regionally restricted	3L	Expression is in smooth muscle cells.
TS24: stomach	Present	Regionally restricted	3L	Expression is in smooth muscle cells.

Structure	Level	Pattern	Image	Note
TS24: lung	Present	Regionally restricted	3M	Homogeneous expression in peribronchiolar and septal connective tissue.
TS24: blood vessel	Present		3M
TS24: rib	Present	Regionally restricted	3M	Expression is in differentiating bone.

Structure	Level	Pattern	Image	Note
TS21: vibrissa	Present		3N

Structure	Level	Pattern	Image	Note
TS21: cornea	Present		3O
TS21: perioptic mesenchyme	Present		3O

Structure	Level	Pattern	Image	Note
TS21: pinna	Present

Structure	Level	Pattern	Image	Note
TS24: bladder	Present	Regionally restricted		Expression is in smooth muscle cells.
TS24: esophagus	Present	Regionally restricted		Expression is in smooth muscle cells.
TS24: uterine horn	Present	Regionally restricted		Expression is in smooth muscle cells.