Antibody:	QM C-17 antibody
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS13: surface ectoderm	Present		1A	Broad pattern of expression on the surface of the embryo.

Structure	Level	Pattern	Image	Note
TS18: surface ectoderm	Present		1B	Expression on the surface of the embryo; more restricted compared to that seen at E8.5.

Structure	Level	Pattern	Image	Note
TS17: limb ectoderm	Strong		3A;B	High expression within the developing epidermis.

Structure	Level	Pattern	Image	Note
TS17: brain ventricle	Present	Regionally restricted	3C;D	Cytoplasmic expression within the lining of the ventricles of the brain.

Structure	Level	Pattern	Image	Note
TS15: heart	Strong		4B
TS15: blood	Absent		4B	No detectable expression in nucleated red blood cell precursors within the heart.

Structure	Level	Pattern	Image	Note
TS19: heart	Weak		4D	Expression downregulated compared with E9.5.
TS19: blood	Absent		4D	No detectable expression in nucleated red blood cell precursors within the heart.

Structure	Level	Pattern	Image	Note
TS21: blood	Absent			No detectable expression in nucleated red blood cell precursors within the heart.

Structure	Level	Pattern	Image	Note
TS15: nervous system	Present			Authors report expression throughout the nervous system.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present		4F

Structure	Level	Pattern	Image	Note
TS15: somite	Strong	Regionally restricted	4H	High expression in a line of cells within the somites.

Structure	Level	Pattern	Image	Note
TS15: somite	Strong	Regionally restricted	4J	High expression in a line of cells within the somites.

Structure	Level	Pattern	Image	Note
TS15: central nervous system ganglion	Strong

Structure	Level	Pattern	Image	Note
TS22: arm	Strong	Regionally restricted	5A	Strong expression within specific zones of developing cartilage. High expression in medially located chondrocytes; no expression within distal regions.

Structure	Level	Pattern	Image	Note
TS22: humerus cartilage condensation	Strong	Regionally restricted	5B	Strong expression within specific zones of developing cartilage. High expression in medially located chondrocytes; no expression within distal regions.

Structure	Level	Pattern	Image	Note
TS22: ulna cartilage condensation	Strong	Regionally restricted	5C	Strong expression within specific zones of developing cartilage. High expression in medially located chondrocytes; no expression within distal regions.

Structure	Level	Pattern	Image	Note
TS22: limb digit	Strong	Regionally restricted	5D	Strong expression within specific zones of developing cartilage. High expression in medially located chondrocytes; no expression within distal regions.

Structure	Level	Pattern	Image	Note
TS24: skin	Present	Regionally restricted	6A	Highest expression within differentated suprabasal keratinocytes.

Structure	Level	Pattern	Image	Note
TS24: vibrissa epidermal component	Present	Regionally restricted	6B	Highest expression within differentated suprabasal keratinocytes.

Structure	Level	Pattern	Image	Note
TS24: limb skin	Present	Regionally restricted	6C	Highest expression within differentated suprabasal keratinocytes.

Structure	Level	Pattern	Image	Note
TS24: limb skin	Present	Regionally restricted	6D	Highest expression within differentated suprabasal keratinocytes.

Structure	Level	Pattern	Image	Note
TS22: ulna cartilage condensation	Strong	Regionally restricted	7B	High expression within differentiated, nonproliferative chondrocytes within the transition zone, but not within the proliferative and hypertrophic zones.

Structure	Level	Pattern	Image	Note
TS22: forelimb epidermis	Strong	Regionally restricted	7D	High expression in nonproliferating keratinocytes within the suprabasal regions, but not in proliferating basal keratinocytes.