Probe:	fragment A
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Probe was subjected to limited alkaline hydrolysis.

Structure	Level	Pattern	Image	Note
TS28: liver	Present	Uniform
TS28: metanephros	Present	Uniform
TS28: placenta	Present	Uniform

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Strong		4a
TS28: dentate gyrus granule cell layer	Strong		4a
TS28: hippocampus pyramidal cell layer	Strong		4a
TS28: thalamus	Moderate		4a
TS28: entorhinal cortex	Present		4a
TS28: olfactory cortex	Present		4a
TS28: cerebellum white matter	Trace		4a
TS28: cerebral hemisphere white matter	Trace		4a

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Strong		4b
TS28: choroid plexus	Strong		4b
TS28: dentate gyrus granule cell layer	Strong		4b
TS28: hippocampus pyramidal cell layer	Strong		4b
TS28: thalamus	Moderate		4b	The paratenial thalamic nucleus had stornger expression.
TS28: entorhinal cortex	Present		4b
TS28: hypothalamus	Present		4b
TS28: olfactory cortex	Present		4b
TS28: pontine nucleus	Present		4b
TS28: reticular thalamic nucleus	Present		4b
TS28: substantia nigra	Present	Regionally restricted	4b	Expression is in the reticular part.
TS28: midbrain	Present	Regionally restricted	4b	Expression is in the cuneiform nucleus.
TS28: cerebellum molecular layer	Trace		4b
TS28: cerebellum white matter	Trace		4b
TS28: cerebral hemisphere white matter	Trace		4b

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Strong		4c
TS28: cerebellum white matter	Trace		4c

Structure	Level	Pattern	Image	Note
TS28: choroid plexus	Strong		4d
TS28: dentate gyrus granule cell layer	Strong		4d
TS28: hippocampus pyramidal cell layer	Strong		4d

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		5a
TS28: cerebellum white matter	Trace		5a

Structure	Level	Pattern	Image	Note
TS28: hippocampus pyramidal cell layer	Strong		5b

Structure	Level	Pattern	Image	Note
TS28: choroid plexus	Strong		5c

Structure	Level	Pattern	Image	Note
TS28: thalamus	Strong	Regionally restricted	5d	Expression is in neurons, glial cells are poorly labeled.
TS28: choroid plexus	Present	Regionally restricted	5d	Expression is in the epithelial cells.
TS28: brain ependyma	Absent		5d

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Present		5e , 5f	Expression varies depending on the presence of spermatozoa, expression is stronger when spermatozoa are absent.

Structure	Level	Pattern	Image	Note
TS15: embryo	Present	Uniform	8a
TS15: central nervous system	Strong		8a

Structure	Level	Pattern	Image	Note
TS19: embryo	Present	Uniform	8b
TS19: neural tube	Strong		8b

Structure	Level	Pattern	Image	Note
TS21: embryo	Present	Uniform	8d
TS21: spinal cord	Strong		8d

Structure	Level	Pattern	Image	Note
TS23: embryo	Present	Uniform	8e
TS23: spinal cord	Moderate		8e

Structure	Level	Pattern	Image	Note
TS25: embryo	Present	Uniform

Structure	Level	Pattern	Image	Note
TS28: mouse	Present	Uniform		The authors state that there is uniform distribution in all tissues.