Gene Expression Data
Assay Details
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Assay
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Reference:
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J:114758
Alcaraz WA, et al., Zfp423 controls proliferation and differentiation of neural precursors in cerebellar vermis formation. Proc Natl Acad Sci U S A. 2006;103(51):19424-19429
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3716188
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Gene symbol:
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Car8
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Gene name:
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carbonic anhydrase 8
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Probe:
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Car8 probe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Specimens were fixed in either 4% paraformaldehyde or 10% formalin, and embedded in 1:1 OCT:Aquamount.
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Results
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Specimen
9 +/+ E15.5 (left):
embryonic day 15.5
(more  )
Specimen
9 +/+ E15.5 (left):
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
9 +/+ E15.5 (center):
embryonic day 15.5
(more )
Specimen
9 +/+ E15.5 (center):
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
9 +/+ E15.5 (right):
embryonic day 15.5
(more )
Specimen
9 +/+ E15.5 (right):
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
9 nur12 E15.5 (left):
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6 * C3H
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Mutant Allele(s):
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Zfp423nur12/Zfp423nur12
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebellum |
Present |
Regionally restricted |
9_nur12 E15.5 (left)
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Expression only in the presumptive cortex, among cells that have migrated out of the ventricular zone.
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Specimen
9 nur12 E15.5 (center):
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6 * C3H
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Mutant Allele(s):
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Zfp423nur12/Zfp423nur12
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebellum |
Present |
Regionally restricted |
9_nur12 E15.5 (center)
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Expression only in the presumptive cortex, among cells that have migrated out of the ventricular zone.
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Specimen
9 nur12 E15.5 (right):
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6 * C3H
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Mutant Allele(s):
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Zfp423nur12/Zfp423nur12
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebellum |
Present |
Regionally restricted |
9_nur12 E15.5 (right)
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Expression only in the presumptive cortex, among cells that have migrated out of the ventricular zone.
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Specimen
9 +/+ P2:
postnatal day 2
(more )
Specimen
9 +/+ P2:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: cerebellum |
Present |
Regionally restricted |
9_+/+ P2
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Expression in Purkinje cells.
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Specimen
9 nur12 P2:
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6 * C3H
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Mutant Allele(s):
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Zfp423nur12/Zfp423nur12
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: cerebellum |
Present |
Regionally restricted |
9_nur12 P2
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Purkinje cells form a less compact zone than in littermate control.
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Specimen
9 +/+ Adult (left):
postnatal adult
(more )
Specimen
9 +/+ Adult (left):
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
9 +/+ Adult (right):
postnatal adult
(more )
Specimen
9 +/+ Adult (right):
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
9 nur12 Adult (left):
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6 * C3H
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Mutant Allele(s):
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Zfp423nur12/Zfp423nur12
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cerebellum |
Present |
Regionally restricted |
9_nur12 Adult (left)
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Expression in Purkinje cells; relatively normal cortical layering in the mutant.
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Specimen
9 nur12 Adult (right):
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6 * C3H
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Mutant Allele(s):
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Zfp423nur12/Zfp423nur12
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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