Gene Expression Data
Assay Details
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Assay
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Reference:
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J:114758
Alcaraz WA, et al., Zfp423 controls proliferation and differentiation of neural precursors in cerebellar vermis formation. Proc Natl Acad Sci U S A. 2006;103(51):19424-19429
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3716183
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Gene symbol:
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Zfp423
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Gene name:
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zinc finger protein 423
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Probe:
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Zfp423 probe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Specimens were fixed in either 4% paraformaldehyde or 10% formalin, and embedded in 1:1 OCT:Aquamount.
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Results
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Specimen
3E E10.5:
embryonic day 10.5
(more  )
Specimen
3E E10.5:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
3E E10.5
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Expression in dorsal neural tube of presumptive hindbrain.
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Specimen
3E E12.5:
embryonic day 12.5
(more )
Specimen
3E E12.5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: cerebellum primordium |
Present |
Regionally restricted |
3E E12.5
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Expression is evident in germinal zones of the developing cerebellum as well as other dividing cell populations. Expression in ventricular zone of cerebellar anlage and prominent expression in the rhombic lip.
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Specimen
3E 15.5:
embryonic day 15.5
(more )
Specimen
3E 15.5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebellum |
Present |
Regionally restricted |
3E E15.5
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Expression in the ventricular zone, rhombic lip and EGL.
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Specimen
not shown:
postnatal day 2
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: cerebellum |
Weak |
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Authors report expression in cerebellum is substantially reduced.
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