Dsp RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:122406 Moore-Scott BA, et al., Identification of molecular markers that are expressed in discrete anterior-posterior domains of the endoderm from the gastrula stage to mid-gestation. Dev Dyn. 2007 Jul;236(7):1997-2003
Assay type:	RNA in situ
MGI Accession ID:	MGI:3716118
Gene symbol:	Dsp
Gene name:	desmoplakin

Probe:	Dsp cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Embryos were post-fixed in 4% paraformaldehyde.

Results

3A ©

Specimen 3A: embryonic day 7.5 (more

)
Note: Late-gastrula.

Structure	Level	Pattern	Image	Note
TS11: extraembryonic visceral endoderm	Weak		3A
TS11: embryo endoderm	Present	Regionally restricted	3A	Expression is in the endoderm adjacent to the node.

3B ©

Specimen 3B: embryonic day 7.75 (more

)

Structure	Level	Pattern	Image	Note
TS11: extraembryonic visceral endoderm	Weak		3B
TS11: embryo endoderm	Present	Regionally restricted	3B	Expression is in the endoderm adjacent to the node and in the posterior endoderm.

3B' ©

Specimen 3B': embryonic day 7.75 (more

)

Structure	Level	Pattern	Image	Note
TS11: extraembryonic visceral endoderm	Weak		3B'
TS11: embryo endoderm	Present	Regionally restricted	3B'	Expression is in the endoderm adjacent to the node and in the posterior endoderm.

3C ©

Specimen 3C: embryonic day 8.0 (more

)

Structure	Level	Pattern	Image	Note
TS12: hindgut diverticulum	Present		3C

3C' ©

Specimen 3C': embryonic day 8.0 (more

)

Structure	Level	Pattern	Image	Note
TS12: hindgut diverticulum	Present		3C'

3D ©

Specimen 3D: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: hindgut diverticulum	Present		3D

3D' ©

Specimen 3D': embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: hindgut diverticulum	Present		3D'

3E ©

Specimen 3E: embryonic day 9.0 (more

)

Structure	Level	Pattern	Image	Note
TS14: hindgut diverticulum	Present		3E
TS14: midgut	Present		3E

3F ©

Specimen 3F: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: hindgut	Present	Regionally restricted	3F	Expression is in the endoderm as well as the surrounding mesoderm.
TS15: midgut	Present	Regionally restricted	3F	Expression is in the endoderm as well as the surrounding mesoderm.
TS15: common atrial chamber	Present		3F
TS15: eye	Present		3F
TS15: otocyst	Present		3F
TS15: branchial groove	Present	Regionally restricted	3F	Expression is in the ectoderm.
TS15: oral region	Present	Regionally restricted	3F	Expression is in the ectoderm.
TS15: heart	Present	Regionally restricted	3F	Expression is in the ventricular chamber.

3F' ©

Specimen 3F': embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: hindgut	Present	Regionally restricted	3F'	Expression is in the endoderm as well as the surrounding mesoderm.
TS15: midgut	Present	Regionally restricted	3F'	Expression is in the endoderm as well as the surrounding mesoderm.

3F'' ©

Specimen 3F'': embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: hindgut	Present	Regionally restricted	3F''	Expression is in the endoderm as well as the surrounding mesoderm.
TS15: midgut	Present	Regionally restricted	3F''	Expression is in the endoderm as well as the surrounding mesoderm.

3G ©

Specimen 3G: embryonic day 11.0 (more

)

Structure	Level	Pattern	Image	Note
TS18: branchial arch	Present		3G
TS18: heart	Present		3G
TS18: tail somite	Present		3G
TS18: trunk somite	Present		3G
TS18: cloaca	Present	Regionally restricted	3G	Expression is in the hindgut-cloaca endoderm.
TS18: hindgut	Present	Regionally restricted	3G	Expression is in the hindgut-cloaca endoderm.

3G' ©

Specimen 3G': embryonic day 11.0 (more

)

Structure	Level	Pattern	Image	Note
TS18: branchial arch	Present		3G'
TS18: cloaca	Present	Regionally restricted	3G'	Expression is in the hindgut-cloaca endoderm.
TS18: hindgut	Present	Regionally restricted	3G'	Expression is in the hindgut-cloaca endoderm.

3G'' ©

Specimen 3G'': embryonic day 11.0 (more

)

Structure	Level	Pattern	Image	Note
TS18: branchial arch	Present		3G''

3G''' ©

Specimen 3G''': embryonic day 11.0 (more

)

Structure	Level	Pattern	Image	Note
TS18: cloaca	Present	Regionally restricted	3G'''	Expression is in the hindgut-cloaca endoderm.
TS18: hindgut	Present	Regionally restricted	3G'''	Expression is in the hindgut-cloaca endoderm.

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