Probe:	Tmprss2 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Embryos were post-fixed in 4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS10: embryo	Absent		1A
TS10: extraembryonic visceral endoderm	Present		1A

Structure	Level	Pattern	Image	Note
TS11: definitive endoderm	Present	Regionally restricted	1B	Expression is overlying the node.

Structure	Level	Pattern	Image	Note
TS11: definitive endoderm	Present	Regionally restricted	1B'	Expression is in most cells overlying the node, both in the endoderm and the cells between the endoderm and the node.

Structure	Level	Pattern	Image	Note
TS11: definitive endoderm	Present	Regionally restricted	1C	Expression is overlying the node.

Structure	Level	Pattern	Image	Note
TS12: midgut endoderm	Present		1D

Structure	Level	Pattern	Image	Note
TS12: midgut endoderm	Present		1D'

Structure	Level	Pattern	Image	Note
TS13: hindgut diverticulum endoderm	Present		1E
TS13: midgut endoderm	Present		1E

Structure	Level	Pattern	Image	Note
TS13: hindgut diverticulum endoderm	Present		1E'
TS13: midgut endoderm	Present		1E'

Structure	Level	Pattern	Image	Note
TS14: hindgut diverticulum	Present	Regionally restricted	1F	Expression is in the endoderm.
TS14: midgut	Present	Regionally restricted	1F	Expression is in the endoderm.

Structure	Level	Pattern	Image	Note
TS15: hindgut	Present	Regionally restricted	1G	Expression is throughout the midgut and hindgut endoderm, extending from the presumptive duodenum to the end of the hindgut.
TS15: midgut	Present	Regionally restricted	1G	Expression is throughout the midgut and hindgut endoderm, extending from the presumptive duodenum to the end of the hindgut.

Structure	Level	Pattern	Image	Note
TS15: hindgut	Present	Regionally restricted	1G'	Expression is throughout the midgut and hindgut endoderm, extending from the presumptive duodenum to the end of the hindgut.
TS15: midgut	Present	Regionally restricted	1G'	Expression is throughout the midgut and hindgut endoderm, extending from the presumptive duodenum to the end of the hindgut.

Structure	Level	Pattern	Image	Note
TS17: hindgut	Present	Regionally restricted	1H	Expression is throughout the midgut and hindgut endoderm, extending from the presumptive duodenum to the end of the hindgut.
TS17: hindgut diverticulum	Present	Regionally restricted	1H	Expression is throughout the midgut and hindgut endoderm, extending from the presumptive duodenum to the end of the hindgut.
TS17: midgut	Present	Regionally restricted	1H	Expression is throughout the midgut and hindgut endoderm, extending from the presumptive duodenum to the end of the hindgut.

Structure	Level	Pattern	Image	Note
TS17: hindgut	Present	Regionally restricted	1H'	Expression is throughout the midgut and hindgut endoderm, extending from the presumptive duodenum to the end of the hindgut.
TS17: hindgut diverticulum	Present	Regionally restricted	1H'	Expression is throughout the midgut and hindgut endoderm, extending from the presumptive duodenum to the end of the hindgut.
TS17: midgut	Present	Regionally restricted	1H'	Expression is throughout the midgut and hindgut endoderm, extending from the presumptive duodenum to the end of the hindgut.