Reporter:	lacZ
	Direct Detection
Assay notes:	Tissue was fixed in 2% formaldehyde, 0.2% glutaldehyde buffer for 10 minutes.

Structure	Level	Pattern	Image	Note
TS27: heart	Strong	Regionally restricted	2B	Expression is present in the fibrous continuity between the mitral and aortic valvue leaflets. Expression patterns are similar to that seen in homozygotes but are less intense.
TS27: bronchus	Present		2B
TS27: trachea	Present		2B

Structure	Level	Pattern	Image	Note
TS27: heart	Strong	Regionally restricted	2C	Expression is present in the fibrous continuity between the mitral and aortic valvue leaflets.
TS27: pulmonary artery	Present		2C

Structure	Level	Pattern	Image	Note
TS27: heart	Present	Regionally restricted	4A	Expression is present in the fibrous continuity of the heart.
TS27: mitral valve	Present		4A	Expression is present in the leaflets.
TS27: tricuspid valve	Present		4A	Expression is present in the leaflets.
TS27: visceral serosal pericardium	Present	Single cells	4A , 4B	Expression is present in a cluster of cells in the epicardial tissue of the terminal groove.
TS27: right atrium	Present	Single cells	4A , 4B	Expression is present in dispersed cells in the epicardial tissue of the right atrium.

Structure	Level	Pattern	Image	Note
TS27: heart	Strong	Regionally restricted	4C	There is strong staining in the fibrous continuity. There are generally high levels of expression in a field of cells extending from the medial cusp of the tricuspid valvue into the anterior leaflet of the mitral valve.

Structure	Level	Pattern	Image	Note
TS27: aortic valve	Present		4E , 4D	Expression is present in the leaflets.
TS27: pulmonary artery	Present	Single cells	4E , 4D	There are strongly staining, but interspersed cells, within the smooth muscle layer of the pulmonary artery.

Structure	Level	Pattern	Image	Note
TS28: aortic valve	Present		5A , 5B	Expression levels are diminished compared to what was seen at neonatal day 2.
TS28: pulmonary valve	Present		5A , 5B	Expression levels are not diminished compared to what was seen in at neonatal day 2.
TS28: pulmonary artery	Present		5A , 5B

Structure	Level	Pattern	Image	Note
TS28: heart	Present	Regionally restricted	5C	Isolated cells close to the aortic valve show staining.
TS28: aortic valve	Present	Single cells	5C , 5D	There is expression in a few cells at the level of the aortic valve leaflets.

Structure	Level	Pattern	Image	Note
TS19: heart right ventricle	Strong		6A
TS19: heart	Strong	Regionally restricted	6A	There is strong staining in the wall of the primary heart tube.
TS19: outflow tract	Present		6A