Probe:	Gpr85 cDNA1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS19: central nervous system	Present	Regionally restricted	1A E11.5	Expression extends in caudal to rostral direction.

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present	Regionally restricted	1A E12.5	Expression extends in caudal to rostral direction.

Structure	Level	Pattern	Image	Note
TS22: central nervous system	Present	Regionally restricted	1A E14.5	Expression extends in caudal to rostral direction.
TS22: cortical plate	Present		1A E14.5

Structure	Level	Pattern	Image	Note
TS24: central nervous system	Present	Regionally restricted	1A E16.5	Expression extends in caudal to rostral direction.
TS24: dorsal root ganglion	Weak		1A E16.5

Structure	Level	Pattern	Image	Note
TS26: central nervous system	Present	Regionally restricted	1A E18.5	Expression extends in caudal to rostral direction.
TS26: cerebral cortex	Strong	Regionally restricted	1A E18.5	Expression is in the outermost layer.
TS26: caudate-putamen	Present	Regionally restricted	1A E18.5
TS26: cerebral cortex intermediate zone	Present	Regionally restricted	1A E18.5
TS26: hypothalamus	Present	Regionally restricted	1A E18.5	Expression is in the hypothalamic nucleus.
TS26: cerebellum	Present	Regionally restricted	1A E18.5	Expression is in the Purkinje cell layer and the deep cerebellar nuclei.
TS26: tectum	Present	Regionally restricted	1A E18.5	Expression is in the inferior colliculus.
TS26: dorsal root ganglion	Weak		1A E18.5

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Present		1B
TS17: dorsal aorta	Present		1B
TS17: future spinal cord	Present		1B
TS17: hindbrain	Present		1B
TS17: midbrain	Present		1B
TS17: telencephalon	Present	Regionally restricted	1B	Expression extended at lower levels to the basal telencephalon.
TS17: trunk mesenchyme	Weak	Regionally restricted	1B	Expression is in smooth and skeletal muscle.
TS17: cardiac muscle tissue	Weak		1B

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	1C	Expression is in the ventral horn and is absent in the ventricular zones.

Structure	Level	Pattern	Image	Note
TS19: hindbrain lateral wall	Present	Regionally restricted	1D	Expression is areas where differentiating cells reside and is absent in the ventricular zones.

Structure	Level	Pattern	Image	Note
TS22: cortical plate	Present		1E

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Strong		1F	Expression is in the outermost layer.
TS26: caudate-putamen	Present		1F
TS26: cerebral cortex intermediate zone	Present		1F
TS26: hypothalamus	Present	Regionally restricted	1F	Expression is in the hypothalamic nucleus.
TS26: tectum	Present	Regionally restricted	1F	Expression is in the inferior colliculus.
TS26: cerebellum	Present	Regionally restricted	1F	Expression is in the Purkinje cell layer and the deep cerebellar nuclei.

Structure	Level	Pattern	Image	Note
TS22: cortical plate	Present		1G

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Strong		1H	Expression is in the outermost layer.
TS24: cerebral cortex intermediate zone	Present		1H

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Strong		1I	Expression is in the outermost layer.
TS26: cerebral cortex intermediate zone	Present		1I

Structure	Level	Pattern	Image	Note
TS24: dorsal root ganglion	Present		2A/A'

Structure	Level	Pattern	Image	Note
TS24: sympathetic ganglion	Present		2B/B'

Structure	Level	Pattern	Image	Note
TS24: enamel organ	Weak		2C/C'

Structure	Level	Pattern	Image	Note
TS28: central nervous system	Weak		3A
TS28: dentate gyrus	Present		3A
TS28: hippocampus pyramidal cell layer	Present		3A

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		3B
TS28: cortical layer III	Present		3B
TS28: cortical layer IV	Present		3B
TS28: cortical layer V	Present		3B
TS28: cortical layer VI	Present		3B
TS28: dentate gyrus	Present		3B
TS28: hippocampus pyramidal cell layer	Present		3B
TS28: cerebral cortex	Very strong	Regionally restricted	3B	Experession is in the induseum grisium.
TS28: paraventricular thalamic nucleus	Strong		3B

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		3C
TS28: cerebellum granule cell layer	Present	Single cells	3C

Structure	Level	Pattern	Image	Note
TS28: adrenal medulla	Present		3D

Structure	Level	Pattern	Image	Note
TS18: trunk mesenchyme	Weak			Expression is in smooth and skeletal muscle.
TS18: cardiac muscle tissue	Weak