Gene Expression Data
Assay Details
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Assay
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Reference:
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J:121844
Zhang FP, et al., An adenosine triphosphatase of the sucrose nonfermenting 2 family, androgen receptor-interacting protein 4, is essential for mouse embryonic development and cell proliferation. Mol Endocrinol. 2007 Jun;21(6):1430-42
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:3715150
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Gene symbol:
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Rad54l2
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Gene name:
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RAD54 like 2 (S. cerevisiae)
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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At least 5 embryos were analyzed; representative results are shown. Both heterozygous and homozygous embryos were stained.
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Results
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Specimen
2A:
(close  )
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Genetic Background:
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involves: ICR
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Mutant Allele(s):
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Rad54l2Gt(A016E03)Oaj/Rad54l2+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: neural ectoderm |
Present |
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2A
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Expression is present in the neural tube.
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Specimen
2B:
(close )
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Genetic Background:
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involves: ICR
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Mutant Allele(s):
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Rad54l2Gt(A016E03)Oaj/Rad54l2+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: central nervous system |
Present |
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2B
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Expression is mainly detected in the neural tube and limbs.
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| TS15: limb |
Present |
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2B
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Expression is mainly detected in the neural tube and limbs.
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Specimen
2C:
(close )
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Genetic Background:
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involves: ICR
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Mutant Allele(s):
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Rad54l2Gt(A016E03)Oaj/Rad54l2+
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Age:
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embryonic day 10.5
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Age Note:
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Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: central nervous system |
Present |
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2C
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Expression is mainly detected in the neural tube and limbs.
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| TS16: limb |
Present |
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2C
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Expression is mainly detected in the neural tube and limbs.
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Specimen
2D:
(close )
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Genetic Background:
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involves: ICR
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Mutant Allele(s):
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Rad54l2Gt(A016E03)Oaj/Rad54l2+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: central nervous system |
Present |
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2D
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Expression is mainly detected in the neural tube and limbs.
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| TS20: limb |
Present |
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2D
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Expression is mainly detected in the neural tube and limbs.
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Specimen
2E:
(close )
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Genetic Background:
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involves: ICR
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Mutant Allele(s):
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Rad54l2Gt(A016E03)Oaj/Rad54l2+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
Not shown:
(close )
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Genetic Background:
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involves: ICR
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Mutant Allele(s):
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Rad54l2Gt(A016E03)Oaj/Rad54l2+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: embryo |
Present |
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From E14.5 onward, expression is virtually ubiquitous.
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Specimen
S1 A:
embryonic day 10.5; Rad54l2Gt(A016E03)Oaj/Rad54l2 +
(more  )
Note: Double labeled: blue - Rad54l2 reporter; green - Rad54l2 protein (assay MGI:3715147).
Specimen
S1 A:
(close )
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Genetic Background:
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involves: ICR
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Mutant Allele(s):
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Rad54l2Gt(A016E03)Oaj/Rad54l2+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: blue - Rad54l2 reporter; green - Rad54l2 protein (assay MGI:3715147).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: central nervous system |
Present |
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S1_A
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Consistent with the immunostaining results, expression is mainly detected in the neural tube and limbs.
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| TS17: limb |
Present |
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S1_A
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Consistent with the immunostaining results, expression is mainly detected in the neural tube and limbs.
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Analysis Tools