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Gene Expression Data
Assay Details
Assay
Reference: J:45214 Dolle P, et al., Differential expression of genes encoding alpha, beta and gamma retinoic acid receptors and CRABP in the developing limbs of the mouse. Nature. 1989 Dec 7;342(6250):702-5
Assay type: RNA in situ
MGI Accession ID: MGI:3713205
Gene symbol: Crabp1
Gene name: cellular retinoic acid binding protein I
Probe: Crabp-1 cDNA1
Probe preparation: Antisense labelled with S35 RNA
Visualized with: Autoradiography
Results
Specimen 1a: embryonic day 10.0 (more )
Note: This is a transverse section.
Structure Level Pattern Image Note
TS16: forelimb bud Present Non-Uniform 1a CRABP Expression is abundant and uniformly distributed in the posterior of the forelimb. There is a gradual proximal to distal increase in transcripts in the anterior. No labelling is seen in the proximal core of the bud; it is maximal at the distal tip.

Specimen 1b: embryonic day 10.0 (more )
Note: This is a transverse section.
Structure Level Pattern Image Note
TS16: forelimb bud Present Non-Uniform 1c CRABP , 1b CRABP Expression is abundant and uniformly distributed in the posterior of the forelimb. There is a gradual proximal to distal increase in transcripts in the anterior. No labelling is seen in the proximal core of the bud; it is maximal at the distal tip.
TS16: forelimb bud apical ectodermal ridge Present 1c CRABP , 1b CRABP The dorsal most cells are labeled; the rest of the limb ectoderm is not.

Specimen 1d: embryonic day 12.5 (more )
Note: This is a longitudinal section.
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Present Non-Uniform 1d CRABP Expression is most abundant in cells underlying the ectoderm and surrounding the limb axial blood vessels; there is very little in the distal mesenchyme.
TS20: forelimb pre-cartilage condensation Absent 1d CRABP

Specimen 1e: embryonic day 12.5 (more )
Note: This is a longitudinal section.
Structure Level Pattern Image Note
TS20: hindlimb mesenchyme Present Non-Uniform 1e CRABP Expression is most abundant in cells underlying the ectoderm; there is very little in the distal mesenchyme.
TS20: hindlimb pre-cartilage condensation Absent 1e CRABP

Specimen 2a: embryonic day 14.5 (more )
Note: This is a sagittal section.
Structure Level Pattern Image Note
TS22: forelimb cartilage condensation Present Regionally restricted 2d CRABP , 2a CRABP Expression is present at the periphery of cartilaginous bone models.
TS22: forelimb dermis Present 2d CRABP , 2a CRABP
TS22: forelimb epidermis Absent 2a CRABP , 2d CRABP
TS22: forelimb musculature Absent 2d CRABP , 2a CRABP
TS22: hand mesenchyme Present Regionally restricted 2a CRABP , 2d CRABP Expression is present in areas where Rarb (see assay MGI:3713206) and Rarg (see assay MGI:3713204) are not maximally expressed.

Specimen 2b: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: hindlimb cartilage condensation Present Regionally restricted 2b CRABP Expression is present at the periphery of cartilaginous bone models.
TS22: hindlimb dermis Present 2b CRABP
TS22: forelimb epidermis Absent 2b CRABP
TS22: hindlimb musculature Absent 2b CRABP
TS22: foot mesenchyme Present 2b CRABP Expression is present in areas where Rarb (see assay MGI:3713206) and Rarg (see assay MGI:3713204) are not maximally expressed.

Specimen 2c: embryonic day 14.5 (more )
Note: This is a transverse section.
Structure Level Pattern Image Note
TS22: hand mesenchyme Present Regionally restricted 2c CRABP Expression is present in areas where Rarb (see assay MGI:3713206) and Rarg (see assay MGI:3713204) are not maximally expressed.


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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/23/2024
MGI 6.23
The Jackson Laboratory