Gene Expression Data
Assay Details
Assay
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Reference:
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J:45214
Dolle P, et al., Differential expression of genes encoding alpha, beta and gamma retinoic acid receptors and CRABP in the developing limbs of the mouse. Nature. 1989 Dec 7;342(6250):702-5
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3713205
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Gene symbol:
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Crabp1
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Gene name:
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cellular retinoic acid binding protein I
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Probe:
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Crabp-1 cDNA1
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
1a:
embryonic day 10.0
(more )
Note: This is a transverse section.
Specimen
1a:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This is a transverse section.
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Structure |
Level |
Pattern |
Image |
Note |
TS16: forelimb bud |
Present |
Non-Uniform |
1a CRABP
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Expression is abundant and uniformly distributed in the posterior of the forelimb. There is a gradual proximal to distal increase in transcripts in the anterior. No labelling is seen in the proximal core of the bud; it is maximal at the distal tip.
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Specimen
1b:
embryonic day 10.0
(more )
Note: This is a transverse section.
Specimen
1b:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Paraffin
|
Note:
|
This is a transverse section.
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: forelimb bud |
Present |
Non-Uniform |
1c CRABP
,
1b CRABP
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Expression is abundant and uniformly distributed in the posterior of the forelimb. There is a gradual proximal to distal increase in transcripts in the anterior. No labelling is seen in the proximal core of the bud; it is maximal at the distal tip.
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TS16: forelimb bud apical ectodermal ridge |
Present |
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1c CRABP
,
1b CRABP
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The dorsal most cells are labeled; the rest of the limb ectoderm is not.
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Specimen
1d:
embryonic day 12.5
(more )
Note: This is a longitudinal section.
Specimen
1d:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
This is a longitudinal section.
|
Specimen
1e:
embryonic day 12.5
(more )
Note: This is a longitudinal section.
Specimen
1e:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This is a longitudinal section.
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Specimen
2a:
embryonic day 14.5
(more )
Note: This is a sagittal section.
Specimen
2a:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
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Not Specified
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Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
This is a sagittal section.
|
Specimen
2b:
embryonic day 14.5
(more )
Specimen
2b:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2c:
embryonic day 14.5
(more )
Note: This is a transverse section.
Specimen
2c:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
This is a transverse section.
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Structure |
Level |
Pattern |
Image |
Note |
TS22: hand mesenchyme |
Present |
Regionally restricted |
2c CRABP
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Expression is present in areas where Rarb (see assay MGI:3713206) and Rarg (see assay MGI:3713204) are not maximally expressed.
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