Probe:	Krox-20 probe2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Similar results were obtained using a 5' probe (MGI:3712817).

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	1a/b	Expression is present in the vicinity of two invaginations of the neuroepithelium termed proneuromeres A and B. Each expressing site is 8-10 cells in length.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	1c/d	This site of expression is in proneuromere B. It extends throughout the neuroepithelium.
TS13: future hindbrain floor plate	Absent		1c/d

Structure	Level	Pattern	Image	Note
TS15: rhombomere 3	Present	Regionally restricted	1e/f	Areas of expression coincide with the sulci separating neuromeres.
TS15: rhombomere 5	Present	Regionally restricted	1e/f , 1g/h	Areas of expression coincide with the sulci separating neuromeres.
TS15: rhombomere 1	Absent		1e/f
TS15: rhombomere 2	Absent		1e/f
TS15: rhombomere 4	Absent		1e/f
TS15: rhombomere 6	Absent		1e/f
TS15: rhombomere 7	Absent		1e/f

Structure	Level	Pattern	Image	Note
TS16: rhombomere 5	Present	Regionally restricted	1i/j	Expression persists in neuromere 5, although it has been down-regulated in neuromere 3.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Trace

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Absent			Authors state that expression was not detected in the CNS at 12.5 days of development.

Structure	Level	Pattern	Image	Note
TS22: hindbrain	Present	Regionally restricted	1k/l	Expression is present in two columns of cells that extend from the level of the vestibular ganglion to the glossopharyngeal ganglion.

Structure	Level	Pattern	Image	Note
TS22: hindbrain	Weak	Regionally restricted	1m/n	Only low levels of expression are detected immediately caudal to the glossopharyngeal ganglion.

Structure	Level	Pattern	Image	Note
TS22: hindbrain	Present	Regionally restricted	1o/p	Expression is present in a ventro-medial column that extends from the level of the vagal ganglion to the caudal end of the hindbrain.

Structure	Level	Pattern	Image	Note
TS24: central nervous system	Absent

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	2a/b	Expression is present in the dorsal neural epithelium, where neural crest cells originate.

Structure	Level	Pattern	Image	Note
TS13: migrating neural crest	Present	Regionally restricted	2c/d	Expression is present in early migratory neural crest cells.

Structure	Level	Pattern	Image	Note
TS17: trigeminal ganglion	Present	Regionally restricted	2e/f	Expression is present in the boundary cap cells. (Boundary caps are comprised of neural crest-derived glial cells that are located adjacent to the neuroepithelium at the sites of its penetration by sensory afferent and motor efferent axons.)

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present	Regionally restricted	2g/h	Expression is present in the boundary cap cells. (Boundary caps are comprised of neural crest-derived glial cells that are located adjacent to the neuroepithelium at the sites of its penetration by sensory afferent and motor efferent axons.)
TS17: facial ganglion	Present	Regionally restricted	2g/h	Expression is present in the boundary cap cells. (Boundary caps are comprised of neural crest-derived glial cells that are located adjacent to the neuroepithelium at the sites of its penetration by sensory afferent and motor efferent axons.)
TS17: trigeminal ganglion	Present	Regionally restricted	2g/h	Expression is present in the boundary cap cells. (Boundary caps are comprised of neural crest-derived glial cells that are located adjacent to the neuroepithelium at the sites of its penetration by sensory afferent and motor efferent axons.)

Structure	Level	Pattern	Image	Note
TS20: cranial ganglion	Present	Regionally restricted	2i/j	Expression is present in the boundary cap cells of all cranial ganglia. (Boundary caps are glial cells that are located adjacent to the neuroepithelium at the sites of its penetration by sensory afferent and motor afferent axons.)

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Present	Regionally restricted	2k/l	Expression is present in the sensory and motor roots of the dorsal root ganglia of the spinal cord.