Antibody:	anti-CT-1
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS13: cardiac muscle tissue	Present		2B
TS13: heart	Present		2B	Expression is present in the looped heart tube. There is no detectable expression in the endocardium or developing endocardial cushions.
TS13: common atrial chamber	Present	Regionally restricted	2B	Expression is present in the muscular portion of the atrial and ventricular chambers.
TS13: primitive ventricle	Present	Regionally restricted	2B	Expression is present in the muscular portion of the atrial and ventricular chambers.
TS28: placenta	Present		2B
TS13: neural ectoderm	Trace		2B	Expression is at near background levels in the developing neural plate.
TS13: 1st branchial arch	Trace		2B	Expression is at near background levels.
TS13: neural crest	Trace		2B	Expression is at near background levels.
TS13: somite	Trace		2B	Expression is at near background levels.
TS13: foregut diverticulum	Weak		2B
TS13: arterial system	Weak	Regionally restricted	2B	Weak expression is present in the aortic sac and the branchial artery from the aortic sac.

Structure	Level	Pattern	Image	Note
TS15: cardiac muscle tissue	Present		2D
TS15: heart	Present		2D	Expression is present in the looped heart tube.
TS28: placenta	Present		2D
TS15: central nervous system	Trace		2D	Expression is near background in the neural plate, neural crest, or neural epithelium.
TS15: trunk somite	Trace		2D

Structure	Level	Pattern	Image	Note
TS17: heart	Present			At this age, expression is predominently in the heart.

Structure	Level	Pattern	Image	Note
TS19: heart	Present		3B	Expression is present in the ventricular compact zone and in trabecular muscle cells.
TS19: endocardial lining	Absent		3B
TS19: outflow tract	Absent		3B
TS19: dorsal root ganglion	Present		3B
TS19: liver	Weak		3B

Structure	Level	Pattern	Image	Note
TS23: atrium cardiac muscle	Strong		3D
TS23: heart	Strong		3D
TS23: ventricle cardiac muscle	Strong		3D
TS23: endocardial cushion	Absent		3D	Expression is not above background.
TS23: aorta	Absent		3D	Expression is not above background in the aortic outflow tract.
TS23: pericardium	Weak		3D
TS23: interventricular septum	Strong		3D
TS23: spinal cord	Present		3D

Structure	Level	Pattern	Image	Note
TS19: brain	Absent
TS19: future neurohypophysis	Absent
TS19: future spinal cord	Absent
TS19: dorsal root ganglion	Present
TS19: oral region gland	Absent			There is no expression in the thymus gland.
TS19: pre-cartilage condensation	Present
TS19: tongue	Present
TS19: heart atrium	Strong
TS19: heart ventricle	Strong
TS19: endocardial cushion	Absent
TS19: outflow tract	Absent
TS19: arterial system	Present	Regionally restricted		Expression is present in the smooth muscle of the arterial vasculature.
TS19: embryo mesenchyme	Present	Regionally restricted		Expression is present in the skeletal muscle.
TS19: liver	Absent
TS19: surface ectoderm	Present			Expression is present in the skin.
TS19: lung epithelium	Absent
TS19: lung mesenchyme	Ambiguous			Expression may or may not be present in the smooth muscle cells of the lung.
TS19: intestine epithelium	Absent
TS19: intestine mesenchyme	Present			Expression is present in the smooth muscle cells of the intestine.

Structure	Level	Pattern	Image	Note
TS21: brain	Ambiguous			Expression may or may not be present in the brain.
TS21: choroid plexus	Absent
TS21: pituitary gland	Absent
TS21: spinal cord	Absent
TS21: dorsal root ganglion	Present
TS21: thymus primordium	Absent
TS21: cartilage	Present
TS21: esophagus	Present
TS21: heart atrium	Strong
TS21: heart ventricle	Strong
TS21: tongue	Strong
TS21: endocardial cushion	Absent
TS21: outflow tract	Absent
TS21: arterial system	Present	Regionally restricted		Expression is present in the smooth muscle of the arterial vasculature.
TS21: skeletal muscle	Present
TS21: gland	Present	Regionally restricted		Expression is present in the adrenal gland.
TS21: liver	Present
TS21: skin	Present
TS21: lung epithelium	Absent
TS21: lung mesenchyme	Present			Expression is present in the smooth muscle cells of the lung.
TS21: intestine epithelium	Absent
TS21: intestine mesenchyme	Present			Expression is present in the smooth muscle cells of the intestine.

Structure	Level	Pattern	Image	Note
TS23: brain	Present
TS23: pituitary gland	Absent
TS23: choroid plexus	Present
TS23: spinal cord	Present
TS23: dorsal root ganglion	Strong
TS23: thymus primordium	Absent
TS23: esophagus	Present
TS23: cartilage	Strong
TS23: heart atrium	Strong
TS23: heart ventricle	Strong
TS23: tongue	Strong
TS23: endocardial cushion	Absent
TS23: aorta	Absent			There is no expression in the outflow tract of the heart.
TS23: arterial system	Present	Regionally restricted		Expression is present in the smooth muscle cells of the arterial vasculature.
TS23: adrenal gland	Strong
TS23: skeletal muscle	Strong
TS23: metanephros	Present
TS23: liver	Strong
TS23: skin	Strong
TS23: lung epithelium	Absent
TS23: lung mesenchyme	Present			Expression is present in the smooth muscle cells of the lung.
TS23: intestine epithelium	Ambiguous			Expression may or may not be present in the mucosal epithelium of the intestine.
TS23: intestine mesenchyme	Present			Expression is present in the smooth muscle cells of the intestine.
TS23: testis	Present

Structure	Level	Pattern	Image	Note
TS23: atrium cardiac muscle	Strong		4A
TS23: ventricle cardiac muscle	Strong		4A
TS23: heart valve	Absent		4A
TS23: aorta	Absent		4A	There is no expression in the outflow tract of the heart.

Structure	Level	Pattern	Image	Note
TS23: atrium cardiac muscle	Strong		4B
TS23: ventricle cardiac muscle	Strong		4B
TS23: heart valve	Absent		4B

Structure	Level	Pattern	Image	Note
TS23: choroid plexus	Present		4C
TS23: midbrain	Ambiguous		4C	There is either weak or negative staining in the midbrain.
TS23: cerebral cortex	Weak		4C	There is weak expression in the cortex of the brain.
TS23: trachea epithelium	Absent		4C

Structure	Level	Pattern	Image	Note
TS23: lung mesenchyme	Present		4D	Expression is present in the smooth muscle cells.

Structure	Level	Pattern	Image	Note
TS23: diaphragm	Strong		4E
TS23: tongue	Strong		4E

Structure	Level	Pattern	Image	Note
TS23: liver	Strong		4F
TS23: intestine mesenchyme	Present		4F	Expression is present in the smooth muscle cells.

Structure	Level	Pattern	Image	Note
TS23: adrenal gland	Present		4G
TS23: renal cortex	Weak		4G

Structure	Level	Pattern	Image	Note
TS23: cartilage	Strong		4H
TS23: dorsal root ganglion	Present		4H

Structure	Level	Pattern	Image	Note
TS23: thymus primordium	Absent		4I