Probe:	Papss2 cDNA fragment 1
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	It is not clear which probe was used or if a mixture of probes was used. The data are annotated with the probe that covers the 3' untranslated region and translated sequences. The other probe; Papss2 cDNA fragment 2 (MGI:3711924) covers the 3' untranslated region.

Structure	Level	Pattern	Image	Note
TS19: future arm mesenchyme	Present	Regionally restricted	2A	Expression is in the cartilagenous condensations such as the humerus and ulna.
TS19: future leg mesenchyme	Weak	Regionally restricted	2A	Expression is in the cartilagenous condensations.
TS19: footplate	Weak	Regionally restricted	2A	Expression is in the digits.
TS19: handplate	Weak	Regionally restricted	2A	Expression is in the digits.
TS19: skeleton	Weak	Regionally restricted	2A	Expression is in the scapula.
TS19: nose	Present	Regionally restricted	2A	Expression is in nasal cartilage.

Structure	Level	Pattern	Image	Note
TS20: cartilage	Strong		2B
TS20: humerus pre-cartilage condensation	Strong		2B
TS20: limb pre-cartilage condensation	Strong		2B
TS20: radius-ulna pre-cartilage condensation	Strong		2B
TS20: vertebral cartilage condensation	Strong		2B
TS20: inner ear	Strong	Regionally restricted	2B	Expression is in the otic vesicle.
TS20: nose	Strong	Regionally restricted	2B	Expression is in nasal cartilage.
TS20: skeleton	Strong	Regionally restricted	2B	Expression is in the scapula.

Structure	Level	Pattern	Image	Note
TS21: Meckel's cartilage	Strong		2C
TS21: cartilage	Strong		2C
TS21: femur cartilage condensation	Strong		2C
TS21: forelimb phalanx pre-cartilage condensation	Strong		2C
TS21: hindlimb phalanx pre-cartilage condensation	Strong		2C
TS21: humerus cartilage condensation	Strong		2C
TS21: rib cartilage condensation	Strong		2C
TS21: nose	Strong	Regionally restricted	2C	Expression is in nasal cartilage.
TS21: brain	Present		2C

Structure	Level	Pattern	Image	Note
TS21: heart	Present	Regionally restricted	2D	Expression is in the endocardial cells.

Structure	Level	Pattern	Image	Note
TS21: tongue	Present	Regionally restricted	2E	Expression is in the connective tissue of the tongue.

Structure	Level	Pattern	Image	Note
TS21: basioccipital pre-cartilage condensation	Present	Regionally restricted	2F	Expression is in the proliferating and prehypertrophic cartilage of the basioccipital bone and is absent in hypertrophic chondrocytes.

Structure	Level	Pattern	Image	Note
TS21: phalanx pre-cartilage condensation	Present		2G

Structure	Level	Pattern	Image	Note
TS24: tail vertebral cartilage condensation	Present		2H

Structure	Level	Pattern	Image	Note
TS24: rib	Present		2I

Structure	Level	Pattern	Image	Note
TS24: basioccipital bone	Present		2K

Structure	Level	Pattern	Image	Note
TS24: hindlimb phalanx	Present		2L

Structure	Level	Pattern	Image	Note
TS24: Meckel's cartilage	Present		2M
TS24: basioccipital bone	Present		2M
TS24: cartilage	Present		2M
TS24: hyoid	Present		2M
TS24: sternum	Present		2M
TS24: thyroid cartilage	Present		2M
TS24: vertebra	Present		2M
TS24: xiphisternum	Present		2M
TS24: nose	Present	Regionally restricted	2M	Expression is in cartilage.
TS24: trachea	Present	Regionally restricted	2M	Expression is in cartilage.

Structure	Level	Pattern	Image	Note
TS24: dorsal root ganglion	Present		2N

Structure	Level	Pattern	Image	Note
TS24: cortical early proximal tubule	Present		2O

Structure	Level	Pattern	Image	Note
TS27: tibia	Present	Regionally restricted	3_Papss2	Expression is in resting, proliferating, and prehypertrophic epiphyseal cartilage, and in the diaphyseal bone.