Antibody:	Anti-OTC
Detection system:	Secondary antibody coupled to Cy3

Structure	Level	Pattern	Image	Note
TS28: small intestine	Present	Regionally restricted	1A	Expression was detected in all epithelial cells except for Paneth cells and goblet cells. There were graduations in intensity with weaker staining in the upper parts of the villus and in the crypts.

Structure	Level	Pattern	Image	Note
TS28: small intestine	Present	Regionally restricted	1C	Expression was detected in all epithelial cells except for Paneth cells and goblet cells.

Structure	Level	Pattern	Image	Note
TS28: small intestine	Present	Regionally restricted	2A	Expression was detected in a mosaic pattern in the heterozygous female. Absorptive cells in the villus portion were aggregates of either positive or negative expression. Crypts were occupied exclusively of positive- or negative-staining cells.

Structure	Level	Pattern	Image	Note
TS28: small intestine	Present	Regionally restricted	2C	Expression was detected in a mosaic pattern in the heterozygous female. Absorptive cells in the villus portion were aggregates of either positive or negative expression. Crypts were occupied exclusively of positive- or negative-staining cells.

Structure	Level	Pattern	Image	Note
TS28: small intestine	Present	Regionally restricted	2E	Expression was detected in a mosaic pattern in the heterozygous female. Absorptive cells in the villus portion were aggregates of either positive or negative expression.

Structure	Level	Pattern	Image	Note
TS23: intestine	Present	Regionally restricted	3A	Expression was detected in all epithelial cells of the small intestine.

Structure	Level	Pattern	Image	Note
TS25: intestine	Present	Regionally restricted	3C	Epithelial cells located in the upper part of the villus of the small intestine were more weakly stained than those in the base region.

Structure	Level	Pattern	Image	Note
TS27: small intestine	Present	Regionally restricted	3E	Expression was detected only in the intervillus region.

Structure	Level	Pattern	Image	Note
TS27: small intestine	Present	Regionally restricted	3G	Expression was detected only in the intervillus region.

Structure	Level	Pattern	Image	Note
TS28: small intestine	Present	Regionally restricted	3H	Expression was detected in the intervillus region and weakly in epithelial cells in the upper part of the villus.

Structure	Level	Pattern	Image	Note
TS28: small intestine	Strong	Regionally restricted	3J	Expression level in the epithelial cells became intense.

Structure	Level	Pattern	Image	Note
TS28: small intestine	Strong	Regionally restricted	3L	Expression level in the epithelial cells became intense.

Structure	Level	Pattern	Image	Note
TS23: intestine	Present	Patchy	4A	Expression was detected in very small patches in the small intestine of the heterozygous mutant and with no definite orientation in the villus structures as seen in the adult.

Structure	Level	Pattern	Image	Note
TS25: intestine	Present	Patchy	4C	Expression was detected in very small patches in the small intestine of the heterozygous mutant and with no definite orientation in the villus structures as seen in the adult. Patches in a villus often connected with those of an adjacent villus.

Structure	Level	Pattern	Image	Note
TS27: small intestine	Present	Patchy	4E	Expression was detected in patches in the presumptive crypts, and the patches were larger than those at E17.5. The crypts were composed of both positive and negative staining cells.

Structure	Level	Pattern	Image	Note
TS27: small intestine	Present	Patchy	4G	Expression was detected in patches in the presumptive crypts, and the patches were larger than those at E17.5. The crypts were composed of both positive and negative staining cells.

Structure	Level	Pattern	Image	Note
TS28: small intestine	Present	Patchy	4I	Expression was detected in patches in the crypts. The crypts were composed of both positive and negative staining cells. Long patches were noted along the villus projection.

Structure	Level	Pattern	Image	Note
TS28: small intestine	Present	Patchy	4K	Expression was detected in patches in the crypts. The crypts were composed of both positive and negative staining cells. Long patches were noted along the villus projection.

Structure	Level	Pattern	Image	Note
TS28: small intestine	Present	Patchy	4M	Expression was detected in the crypts. Crypts mostly contained cells exclusively stained either positive or negative. Some crypts were noted that contained mixed staining. Some small positive patches were also seen near the base part of the villus.

Structure	Level	Pattern	Image	Note
TS28: small intestine	Present	Patchy	4O	Expression was detected in the crypts. Crypts mostly contained cells exclusively stained either positive or negative. Some crypts were noted that contained mixed staining. Some small positive patches were also seen near the base part of the villus.

Structure	Level	Pattern	Image	Note
TS25: intestine	Present	Patchy	5A	Expression was detected in patches in the villus of the small intestine. Fig. 5 was a set of serial sections showing that positive patches in a villus were often connected with those of the adjacent villus, but not always.

Structure	Level	Pattern	Image	Note
TS25: intestine	Present	Patchy	5C	Expression was detected in patches in the villus of the small intestine. Fig. 5 was a set of serial sections showing that positive patches in a villus were often connected with those of the adjacent villus, but not always.

Structure	Level	Pattern	Image	Note
TS25: intestine	Present	Patchy	5E	Expression was detected in patches in the villus of the small intestine. Fig. 5 was a set of serial sections showing that positive patches in a villus were often connected with those of the adjacent villus, but not always.

Structure	Level	Pattern	Image	Note
TS25: intestine	Present	Patchy	5G	Expression was detected in patches in the villus of the small intestine. Fig. 5 was a set of serial sections showing that positive patches in a villus were often connected with those of the adjacent villus, but not always.

Structure	Level	Pattern	Image	Note
TS25: intestine	Present	Patchy	5I	Expression was detected in patches in the villus of the small intestine. Fig. 5 was a set of serial sections showing that positive patches in a villus were often connected with those of the adjacent villus, but not always.