Probe:	clone 1C
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS9: embryo	Absent

Structure	Level	Pattern	Image	Note
TS12: future prosencephalon	Weak	Regionally restricted	3a	There are low levels of expression in the ventral forebrain.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Strong	Regionally restricted	3b	Expression is present in the ventral forebrain.

Structure	Level	Pattern	Image	Note
TS14: somite	Present	Regionally restricted	3c	Expression is present in the dorsal-most region of the somites.
TS14: surface ectoderm	Present	Regionally restricted	3c	Expression is present in the surface ectoderm of the cranial region.
TS14: prosencephalon	Present	Regionally restricted	3c	Expression is present in the ventral forebrain.
TS14: allantois	Present		3c

Structure	Level	Pattern	Image	Note
TS15: trunk dermomyotome	Present	Regionally restricted	3d	Expression is present in the dorsal-most region of the somites.
TS15: surface ectoderm	Present		3d	Expression is seen throughout the surface ectoderm.
TS15: infundibular recess of 3rd ventricle	Present		3d	Expression is present in the infundibulum.

Structure	Level	Pattern	Image	Note
TS17: limb	Present		3e
TS17: trunk dermomyotome	Present		3e
TS17: surface ectoderm	Present		3e	Expression is seen throughout the surface ectoderm.
TS17: infundibular recess of 3rd ventricle	Present		3e	Expression is present in the infundibulum.

Structure	Level	Pattern	Image	Note
TS17: infundibular recess of 3rd ventricle	Strong		3f	There is prominent expression in the infundibulum.

Structure	Level	Pattern	Image	Note
TS17: infundibular recess of 3rd ventricle	Present		3g	There is expression in the infundibulum.
TS17: Rathke's pouch	Absent		3g

Structure	Level	Pattern	Image	Note
TS17: trunk dermomyotome	Present		3h

Structure	Level	Pattern	Image	Note
TS21: hand mesenchyme	Present	Regionally restricted	3i	Expression is present in the digital mesenchyme.

Structure	Level	Pattern	Image	Note
TS19: gonad	Present			Expression is present in the developing gonads of both males and females.

Structure	Level	Pattern	Image	Note
TS19: gonad	Present			Expression is present in the developing gonads of both males and females.

Structure	Level	Pattern	Image	Note
TS21: testis mesenchyme	Present		3j	Expression is present in the interstitial region between the developing sex cords.
TS21: primary sex cord	Absent		3j

Structure	Level	Pattern	Image	Note
TS21: ovary	Present	Uniform		There is uniform expression throughout.

Structure	Level	Pattern	Image	Note
TS21: skin	Present	Regionally restricted		Expression is present in the hair follicles.